| 19225937 |
Hirose S, Momosaki S, Hosoi R, Abe K, Gee A, Inoue O: Role of NMDA receptor upon [14C] acetate uptake into intact rat brain. . Ann Nucl Med. 2009 Feb;23(2):143-7. Epub 2009 Feb 19.
OBJECTIVE: To clarify the role of N-methyl-D: -aspartate (NMDA) receptors upon [(14) C] acetate uptake in the rodent central nervous system (CNS), ibotenic acid (IBO) was infused into the right striatum of the rat brain. METHODS: Autoradiograms of [(14) C] acetate uptake in the brain for 2 h following the infusion of IBO (10 microg/microl) were obtained in both non-treated and MK-801 (1 mg/kg, i.v.) pretreated rats. The effect of MK-801 on [(14) C] acetate uptake in the normal rat brain was also studied. RESULTS: Infusion of IBO significantly decreased [(14) C] acetate uptake in the infused side of the striatum. The expression of monocarboxylate transporter-1 was not altered, suggesting that the activity of tricarboxylic acid (TCA) cycle in glial cells might be depressed. Pretreatment with MK-801 completely blocked the decreasing effect of IBO on [(14) C] acetate uptake. MK-801 also increased [(14) C] acetate uptake in the whole brain of normal rats. CONCLUSIONS: These results indicate the important roles of NMDA receptors on [(14) C] acetate uptake in the intact rat brain. |
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