| 15366887 |
Matsunaga D, Asanuma H, Komiyama M: Photoregulation of RNA digestion by RNase H with azobenzene-tethered DNA. J Am Chem Soc. 2004 Sep 22;126(37):11452-3.
RNA digestion by RNase H, which is responsible for the antisense effect, was efficiently photoregulated by use of the duplex of azobenzene-tethered sense DNA and native antisense DNA. In the dark, RNA digestion was suppressed because antisense DNA was strongly hybridized with azobenzene-tethered sense DNA, and accordingly RNA was isolated. On UV irradiation, antisense DNA was released from the azobenzene-tethered DNA due to the trans-to-cis isomerization and hybridized with RNA, which was digested by RNase H. |
18(0,0,3,3) |