Protein Information

Name casein
Synonyms Alpha; Alpha s1 casein; Alpha s1 casein precursor; CASA; CSN 1; CSN1; CSN1S1; Casein…

Compound Information

Name kinetin
CAS

Reference List

PubMed Abstract RScore(About this table)
14531034 Narula A, Kumar S, Bansal KC, Srivastava PS: In vitro micropropagation, differentiation of aerial bulbils and tubers and diosgenin content in Dioscorea bulbifera. Planta Med. 2003 Aug;69(8):778-9.

The best medium for regeneration and bulbil differentiation was MS + 0.5 microM IAA (indole-3-acetic acid) + 20.0 microM Kn (kinetin) + 500 mg/L CH (casein hydrolysate) + activated charcoal (20 %).
31(0,1,1,1) Details
5762609 Tiwari MM, Arya HC: Sclerospora graminicola axenic culture. Science. 1969 Jan 17;163(864):291-3.

Sclerospora graminicola was first grown on host callus tissue and subsequently on a modification of White's basal medium that contained casein hydrolyzate (Oxoid). 2,4-dichlorophenoxyacetic acid, and kinetin.
31(0,1,1,1) Details
11831375 Kumar R, Chowdhury JB, Jain RK: Interspecific hybridization in Brassica juncea and Brassica tournefortii through embryo rescue and their evaluation for biotic and abiotic stress tolerance. Indian J Exp Biol. 2001 Sep;39(9):911-5.

The best response was observed from culture of embryos excised 20 days after pollination on MS medium supplemented with kinetin, alpha-naphthylacetic acid, gibberellic acid, glutamine and casein hydrolysate.
6(0,0,1,1) Details
7765304 Fett-Neto AG, Melanson SJ, Sakata K, DiCosmo F: Improved growth and taxol yield in developing calli of Taxus cuspidata by medium composition modification. Biotechnology (N Y). 1993 Jun;11(6):731-4.

Several sequential modifications were made to the basal B5 medium, which included addition and/or variation in the concentration of sucrose, B5 organic supplements, gibberellic acid, 36 combinations of 2,4-D/kinetin ratios, media salts and organic supplements, phenylalanine, casein hydrolysate and medium pH.
6(0,0,1,1) Details
14762684 Hou SW, Jia JF: Plant regeneration from protoplasts isolated from embryogenic calli of the forage legume Astragalus melilotoides Pall. Plant Cell Rep. 2004 May;22(10):741-6. Epub 2004 Feb 5.

The highest division frequency (9.86 +/- 0.68%) and plating efficiency (1.68 +/- 0.05%) were obtained in solid-liquid medium (KM8P) supplemented with 1.0 mg/l 2,4-dichlorophenoxyacetic acid, 0.5 mg/l 6-benzylaminopurine (BA), 0.2 mg/l kinetin, 0.2 M glucose, 0.3 M mannitol and 500 mg/l casein hydrolysate.
6(0,0,1,1) Details
16862300 Franco ET, Gavioli LB, Ferreira AG: In vitro regeneration of Didymopanax morototoni. Braz J Biol. 2006 May;66(2A):455-62.

In the first step, woody plant medium (WPM) with casein hydrolysate (250 mgL-1 ) and 2,4-D (1.0 and 5.0 mgL-1) were used combined with kinetin (0.1 and 1.0 mgL-1).
6(0,0,1,1) Details
17238101 Purohit M, Pande D, Datta A, Srivastava PS: Enhanced xanthotoxin content in regenerating cultures of Ammi majus and micropropagation. Planta Med. 1995 Oct;61(5):481-2.

The callus from cotyledonary leaves obtained on MS medium supplemented with alpha-indoleacetic acid (IAA) + kinetin (KN) + casein hydrolysate (CH), differentiated shoots in 100 % cultures when adenine was added to the above medium.
6(0,0,1,1) Details
9581525 Jha S, Sanyal D, Ghosh B, Jha TB: Improved taxol yield in cell suspension culture of Taxus wallichiana (Himalayan yew). Planta Med. 1998 Apr;64(3):270-2.

Cell line NC110 derived from needle leaf of a 40-year-old tree growing in Darjeeling Himalayas produced 0.018% taxol in B5 basal medium supplemented with 2,4-D (2 mg/l), kinetin (0.5 mg/l), and casein hydrolysate (0.5%).
6(0,0,1,1) Details
16850870 Li L, Zhang CR: Production of puerarin and isoflavones in cell suspension cultures of Pueraria lobata (Willd.): effects of medium supplementation with casein hydrolysate and coconut milk. J Environ Biol. 2006 Jan;27(1):21-6.

Callus induced from leaf explants of Pueraria lobata seedlings were suspended in Gamborg B5 medium supplemented with 1 mg l (-1) 2,4-dichlorophenoxyacetic acid, 1 mg l (-1) naphthalene acetic acid, 0.5 mg l (-1) kinetin and 30 g l (-1) sucrose.
2(0,0,0,2) Details
9030781 Meijer L, Borgne A, Mulner O, Chong JP, Blow JJ, Inagaki N, Inagaki M, Delcros JG, Moulinoux JP: Biochemical and cellular effects of roscovitine, a potent and selective inhibitor of the cyclin-dependent kinases cdc2, cdk2 and cdk5. Eur J Biochem. 1997 Jan 15;243(1-2):527-36.


The kinase specificity of roscovitine was investigated with 25 highly purified kinases (including protein kinase A, G and C isoforms, myosin light-chain kinase, casein kinase 2, insulin receptor tyrosine kinase, c-src, v-abl).
1(0,0,0,1) Details
17016734 Walia N, Kaur A, Babbar SB: An efficient, in vitro cyclic production of shoots from adult trees of Crataeva nurvala Buch. Plant Cell Rep. 2007 Mar;26(3):277-84. Epub 2006 Oct 3.


Addition of casein hydrolysate significantly increased the average number of shoots per explant.
2(0,0,0,2) Details
12789423 Yemets AI, Klimkina LA, Tarassenko LV, Blume YB: Efficient callus formation and plant regeneration of goosegrass [Eleusine indica (L.) Gaertn.]. Plant Cell Rep. 2003 Feb;21(6):503-10. Epub 2002 Dec 14.

The optimum medium for inducing morphogenic calli consisted of N6 basal salts and B5 vitamins supplemented with 1-2 mg l (-1) 2,4-dichlorophenoxyacetic acid (2,4-D), 2 mg l (-1) glycine, 100 mg l (-1) asparagine, 100 mg l (-1) casein hydrolysate, 30 g l (-1) sucrose and 0.6% agar, pH 5.7.
Morphogenic structures of direct shoots and somatic embryos were grown into rooted plantlets on medium containing MS basal salts, B5 vitamins, 1 mg l (-1) kinetin (Kn) and 0.1 mg l (-1) indole-3-acetic acid (IAA), 3% sucrose, 0.6% agar, pH 5.7.
1(0,0,0,1) Details
16656573 Penner D, Ashton FM: Hormonal Control of Proteinase Activity in Squash Cotyledons. Plant Physiol. 1967 Jun;42(6):791-796.

A crude proteolytic enzyme preparation which hydrolyzes casein was isolated from the cotyledons of squash seedlings.
The presence of the embryonic axis during the first 32 hours of germination was a prerequisite for the development of maximum proteolytic activity.The presence of a cytokinin, such as benzyladenine, kinetin, BTP [6-(benzylamino)-9-(2-tetrahydropyranyl)-9H-purine], or phenyladenine, in the culture solution could reproduce the effect of the embryonic axis.
1(0,0,0,1) Details
16999034 Deb CR, Temjensangba: In vitro propagation of threatened terrestrial orchid, Malaxis khasiana Soland ex. Indian J Exp Biol. 2006 Sep;44(9):762-6.

Immature seeds of 8-9 week after pollination (WAP) cultured on MS medium (2% sucrose) supplemented with 500 mgl (-1) casein-hydrolysate and 1 microM N6-benzyladenine (BA) exhibited germination of 75% seeds after 107 days of culture and subsequently supported the development of PLBs.
Subsequent culture on MS medium enriched with 6 microM of indole-3-acetic acid (IAA), 18 microM each of BA and kinetin induced multiple shoots and plantlets.
1(0,0,0,1) Details
16129608 Tang W, Newton RJ: Peroxidase and catalase activities are involved in direct adventitious shoot formation induced by thidiazuron in eastern white pine (Pinus strobus L.) zygotic embryos. Plant Physiol Biochem. 2005 Aug;43(8):760-9. Epub 2005 Jun 13.


Among different concentrations of casein enzymatic hydrosylate (CH) and glutamine used in this study, 500 mg l (-1) CH or 600 mg l (-1) glutamine induced the highest frequency of DAS formation.
1(0,0,0,1) Details
12827435 Ipekci Z, Gozukirmizi N: Direct somatic embryogenesis and synthetic seed production from Paulownia elongata. Plant Cell Rep. 2003 Aug;22(1):16-24. Epub 2003 Jun 24.

Several plant growth regulators [6-benzylaminopurine, indole-3-acetic acid, alpha-naphthaleneacetic acid, kinetin and thidiazuron (TDZ)] alone or in combination were tested for their capacity to induce somatic embryogenesis.
The highest induction frequencies of somatic embryos were obtained on Murashige and Skoog (MS) medium supplemented with 3% sucrose, 0.6% Phytagel, 500 mg l (-1) casein hydrolysate and 10 mg l (-1) TDZ (medium MS10).
1(0,0,0,1) Details
8225416 Raval M, Chattoo BB: Role of media constituents and proline in callus growth, somatic embryogenesis and regeneration of Oryza sativa cv Indica. Indian J Exp Biol. 1993 Jul;31(7):600-3.


Cytokinins like BAP or kinetin alone could not initiate shoot formation.
0(0,0,0,0) Details