Protein Information

Name microRNAs
Synonyms MIRN410; hsa mir 410; microRNA; hsa mir 410s; microRNAs

Compound Information

Name formaldehyde
CAS formaldehyde

Reference List

PubMed Abstract RScore(About this table)
18755714 Siebolts U, Varnholt H, Drebber U, Dienes HP, Wickenhauser C, Odenthal M: Tissues from routine pathology archives are suitable for microRNA analyses by quantitative PCR. J Clin Pathol. 2009 Jan;62(1):84-8. Epub 2008 Aug 28.

8(0,0,0,8) Details
18307259 Varnholt H, Drebber U, Schulze F, Wedemeyer I, Schirmacher P, Dienes HP, Odenthal M: MicroRNA gene expression profile of hepatitis C virus-associated hepatocellular carcinoma. Hepatology. 2008 Apr;47(4):1223-32.

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19038028 Hasemeier B, Christgen M, Kreipe H, Lehmann U: Reliable microRNA profiling in routinely processed formalin-fixed paraffin-embedded breast cancer specimens using fluorescence labelled bead technology. BMC Biotechnol. 2008 Nov 27;8:90.

7(0,0,0,7) Details
18403610 Doleshal M, Magotra AA, Choudhury B, Cannon BD, Labourier E, Szafranska AE: Evaluation and validation of total RNA extraction methods for microRNA expression analyses in formalin-fixed, paraffin-embedded tissues. J Mol Diagn. 2008 May;10(3):203-11. Epub 2008 Apr 10.

2(0,0,0,2) Details
18832457 Zhang X, Chen J, Radcliffe T, Lebrun DP, Tron VA, Feilotter H: An array-based analysis of microRNA expression comparing matched frozen and formalin-fixed paraffin-embedded human tissue samples. J Mol Diagn. 2008 Nov;10(6):513-9. Epub 2008 Oct 2.

2(0,0,0,2) Details
19137005 Pena JT, Sohn-Lee C, Rouhanifard SH, Ludwig J, Hafner M, Mihailovic A, Lim C, Holoch D, Berninger P, Zavolan M, Tuschl T: miRNA in situ hybridization in formaldehyde and EDC-fixed tissues. Nat Methods. 2009 Feb;6(2):139-41. Epub 2009 Jan 11.

We showed that in situ hybridization (ISH) using conventional formaldehyde fixation results in substantial microRNA loss from mouse tissue sections, which can be prevented by fixation with 1-ethyl-3-(3-dimethylaminopropyl) carbodiimide that irreversibly immobilizes the microRNA at its 5' phosphate.
7(0,0,1,2) Details
17698639 Xi Y, Nakajima G, Gavin E, Morris CG, Kudo K, Hayashi K, Ju J: Systematic analysis of microRNA expression of RNA extracted from fresh frozen and formalin-fixed paraffin-embedded samples. RNA. 2007 Oct;13(10):1668-74. Epub 2007 Aug 13.

2(0,0,0,2) Details
18383833 Hoefig KP, Thorns C, Roehle A, Kaehler C, Wesche KO, Repsilber D, Branke B, Thiere M, Feller AC, Merz H: Unlocking pathology archives for microRNA-profiling. Anticancer Res. 2008 Jan-Feb;28(1A):119-23.

2(0,0,0,2) Details
19888862 Lassalle S, Hofman V, Marius I, Gavric-Tanga V, Brest P, Havet K, Butori C, Selva E, Santini J, Mograbi B, Hofman P: Assessment of morphology, antigenicity, and nucleic acid integrity for diagnostic thyroid pathology using formalin substitute fixatives. Thyroid. 2009 Nov;19(11):1239-48.

BACKGROUND: With the advent of the formaldehyde standard law in France, and because of the impact of new methods for diagnosis and prognosis in pathology, formalin replacement in surgical pathology laboratories is currently being discussed in France.
The objective of this study was to compare formalin substitute fixation with formalin fixation and cryoconservation of tissues from several benign and malignant thyroid pathologies with respect to morphology, antigenicity, and nucleic acid (RNA, DNA, microRNA) integrity.
2(0,0,0,2) Details
18158131 Nuovo GJ: In situ detection of precursor and mature microRNAs in paraffin embedded, formalin fixed tissues and cell preparations. Methods. 2008 Jan;44(1):39-46.

2(0,0,0,2) Details
19005486 Glud M, Klausen M, Gniadecki R, Rossing M, Hastrup N, Nielsen FC, Drzewiecki KT: MicroRNA expression in melanocytic nevi: the usefulness of formalin-fixed, paraffin-embedded material for miRNA microarray profiling. J Invest Dermatol. 2009 May;129(5):1219-24. Epub 2008 Nov 13.

2(0,0,0,2) Details
19161223 Pearson BJ, Eisenhoffer GT, Gurley KA, Rink JC, Miller DE, Sanchez Alvarado A: Formaldehyde-based whole-mount in situ hybridization method for planarians. Dev Dyn. 2009 Feb;238(2):443-50.

The new method gives robust, high-resolution labeling in fine anatomical detail, allows co-labeling with fluorescent probes, and is sufficiently sensitive to resolve the expression pattern of a microRNA in planarians.
1(0,0,0,1) Details
18687792 Szafranska AE, Davison TS, Shingara J, Doleshal M, Riggenbach JA, Morrison CD, Jewell S, Labourier E: Accurate molecular characterization of formalin-fixed, paraffin-embedded tissues by microRNA expression profiling. J Mol Diagn. 2008 Sep;10(5):415-23. Epub 2008 Aug 7.

1(0,0,0,1) Details
18058265 Tetzlaff MT, Liu A, Xu X, Master SR, Baldwin DA, Tobias JW, Livolsi VA, Baloch ZW: Differential expression of miRNAs in papillary thyroid carcinoma compared to multinodular goiter using formalin fixed paraffin embedded tissues. Endocr Pathol. 2007 Fall;18(3):163-73.


microRNAs (miRNAs) are approximately 22 nt RNAs that negatively regulate target gene expression.
1(0,0,0,1) Details
19710399 Ma Z, Lui WO, Fire A, Dadras SS: Profiling and discovery of novel miRNAs from formalin-fixed, paraffin-embedded melanoma and nodal specimens. J Mol Diagn. 2009 Sep;11(5):420-9.


Formalin-fixed, paraffin-embedded tissue is known to contain a wealth of molecular information in the form of microRNAs (miRNAs), which could be correlated with clinical outcome for improved prognostication and/or treatment response. miRNAs are endogenous, noncoding RNAs ( approximately 22 nucleotides) and may function as tumor suppressors or oncogenes.
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