| 18667429 |
Soragni E, Kassavetis GA: Absolute gene occupancies by RNA polymerase III, TFIIIB, and TFIIIC in Saccharomyces cerevisiae. J Biol Chem. 2008 Sep 26;283(39):26568-76. Epub 2008 Jul 30.
We achieved this by spiking every immunoprecipitation reaction with the formaldehyde cross-linked in vitro counterparts of TFIIIB-, TFIIIC-, and pol III-DNA complexes, measuring the in vitro occupancies of the corresponding factors on a DNA probe and determining probe recovery by quantitative PCR. |
85(1,1,1,5) |
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