Protein Information

Name ribonuclease A
Synonyms HP RNase; Ribonuclease; Ribonuclease A; Pancreatic ribonuclease; Pancreatic ribonuclease precursor; RIB 1; RIB1; RNASE1…

Compound Information

Name formaldehyde
CAS formaldehyde

Reference List

PubMed Abstract RScore(About this table)
18490897 Fowler CB, O'Leary TJ, Mason JT: Modeling formalin fixation and histological processing with ribonuclease A: effects of ethanol dehydration on reversal of formaldehyde cross-links. Lab Invest. 2008 Jul;88(7):785-91. Epub 2008 May 19.
149(1,3,4,4) Details
17938284 Vecchio L, Solimando L, Biggiogera M, Fakan S: Use of halogenated precursors for simultaneous DNA and RNA detection by means of immunoelectron and immunofluorescence microscopy. J Histochem Cytochem. 2008 Jan;56(1):45-55. Epub 2007 Oct 15.

RNase and DNase digestion of ultrathin sections after formaldehyde fixation and acrylic resin embedding confirmed the specificity of incorporation.
6(0,0,1,1) Details
18077427 Tardiff DF, Abruzzi KC, Rosbash M: Protein characterization of Saccharomyces cerevisiae RNA polymerase II after in vivo cross-linking. Proc Natl Acad Sci U S A. 2007 Dec 11;104(50):19948-53. Epub 2007 Dec 5.

To characterize proteins associated with active transcription complexes, we purified RNA polymerase II (pol II) from Saccharomyces cerevisiae after fixing live cells with formaldehyde.
A tagged RNP protein reciprocally purified pol II under identical cross-linking conditions, and the association between RNP proteins and pol II was largely RNase-sensitive.
1(0,0,0,1) Details
17530029 Fowler CB, Cunningham RE, O'Leary TJ, Mason JT: 'Tissue surrogates' as a model for archival formalin-fixed paraffin-embedded tissues. Lab Invest. 2007 Aug;87(8):836-46. Epub 2007 May 28.

However, advances in FFPE tissue-based proteomics have been hampered by inefficient methods to extract proteins from archival tissue and by an incomplete knowledge of formaldehyde-induced modifications in proteins.
Cytoplasmic proteins, such as lysozyme or ribonuclease A, at concentrations approaching the protein content in whole cells, are fixed with 10% formalin to form gelatin-like plugs.
1(0,0,0,1) Details