| Name | caspase 9 |
|---|---|
| Synonyms | APAF 3; APAF3; Apoptotic protease MCH6; Apoptotic protease Mch 6; Apoptotic protease activating factor 3; CASP 9; CASP9; CASPASE 9c… |
| Name | cycloheximide |
|---|---|
| CAS |
| PubMed | Abstract | RScore(About this table) | |
|---|---|---|---|
| 18234961 | Chetoui N, Sylla K, Gagnon-Houde JV, Alcaide-Loridan C, Charron D, Al-Daccak R, Aoudjit F: Down-regulation of mcl-1 by small interfering RNA sensitizes resistant melanoma cells to fas-mediated apoptosis. Mol Cancer Res. 2008 Jan;6(1):42-52. In this study, we report that treatment of Fas-resistant melanoma cell lines with cycloheximide, a general inhibitor of de novo protein synthesis, sensitizes them to anti-Fas monoclonal antibody (mAb)-induced apoptosis. Targeting Mcl-1 in these melanoma cell lines with specific small interfering RNA was sufficient to sensitize them to both anti-Fas mAb-induced apoptosis and activation of caspase-9. |
1(0,0,0,1) | Details |
| 20032379 | Eriksson D, Lofroth PO, Johansson L, Riklund K, Stigbrand T: Apoptotic signalling in HeLa Hep2 cells following 5 Gy of -60 gamma radiation. Anticancer Res. 2009 Nov;29(11):4361-6. The activation of caspase-2, caspase-8, caspase-9 and effector caspase-3 was investigated by caspase assay plates and Western blots. HeLa Hep2 cells were irradiated with or without preincubation with inhibitors of protein synthesis (cycloheximide, CHX) and caspases, followed by TUNEL staining and caspase assay plate evaluation. |
1(0,0,0,1) | Details |
| 18231987 | Stanic I, Cetrullo S, Facchini A, Stefanelli C, Borzi RM, Tantini B, Guarnieri C, Caldarera CM, Flamigni F: Effect of the polyamine analogue N1,N11-diethylnorspermine on cell survival and susceptibility to apoptosis of human chondrocytes. J Cell Physiol. 2008 Jul;216(1):153-61. In conclusion, following DENSPM treatment, C-28/I2 chondrocytes are initially sensitized to caspase 9-dependent apoptosis in the presence of TNF and CHX and may eventually undergo a late and mainly caspase-independent cell death in the absence of other stimuli. |
1(0,0,0,1) | Details |
| 15629764 | Ebert AD, Chen F, He X, Cryns VL, Bohn MC: A tetracycline-regulated adenovirus encoding dominant-negative caspase-9 is regulated in rat brain and protects against neurotoxin-induced cell death in vitro, but not in vivo. Exp Neurol. 2005 Feb;191 Suppl 1:S80-94. Caspase-9 is a critical downstream effector molecule involved in apoptosis, a cell death process thought to be involved in the demise of (DA) neurons in the substantia nigra (SN) affected by Parkinson's disease (PD). We next observed that Casp9DN gene delivery significantly protected against TNFalpha and cycloheximide-induced chromatin condensation in HeLa cells and prevented chromatin condensation and the appearance of the early apoptotic marker annexin V in (6-OHDA) treated MN9D cells, a dopaminergic cell line. |
1(0,0,0,1) | Details |
| 19952118 | Ballot C, Kluza J, Martoriati A, Nyman U, Formstecher P, Joseph B, Bailly C, Marchetti P: Essential role of mitochondria in apoptosis of cancer cells induced by the marine alkaloid Lamellarin D. Mol Cancer Ther. 2009 Dec;8(12):3307-17. Epub . However, lamellarin D killed efficiently mutated p53 or p53 null cancer cells, and sensitivity to lamellarin D was abrogated neither by cycloheximide nor in enucleated cells. The drug induced conformational activation of Bax and decreased the expression levels of antiapoptotic proteins Bcl-2 and cIAP2 in association with activation of caspase-9 and caspase-3. |
1(0,0,0,1) | Details |
| 19453217 | Vinken M, Decrock E, De Vuyst E, Leybaert L, Vanhaecke T, Rogiers V: Biochemical characterisation of an in vitro model of hepatocellular apoptotic cell death. Altern Lab Anim. 2009 Apr;37(2):209-18. This study was set up to critically evaluate a commonly-used in vitro model of hepatocellular apoptotic cell death, in which freshly isolated hepatocytes, cultured in a monolayer configuration, are exposed to a combination of Fas ligand and cycloheximide for six hours. The initiation of apoptosis was evidenced by the activation of caspase 8 and caspase 9, and increased Annexin-V reactivity. |
1(0,0,0,1) | Details |
| 20017956 | Cho SH, Chung KS, Choi JH, Kim DH, Lee KT: Compound K, a metabolite of ginseng saponin, induces apoptosis via caspase-8-dependent pathway in HL-60 human leukemia cells. BMC Cancer. 2009 Dec 18;9:449. Interestingly, the activation of caspase-3 and -8 and DNA fragmentation were significantly prevented in the presence of cycloheximide, suggesting that Compound K-induced apoptosis is dependent on de novo protein synthesis. CONCLUSIONS: The results indicate that caspase-8 plays a key role in Compound K-stimulated apoptosis via the activation of caspase-3 directly or indirectly through Bid cleavage, cytochrome c release, and caspase-9 activation. |
1(0,0,0,1) | Details |
| 17194804 | Lee SY, Cherla RP, Tesh VL: Simultaneous induction of apoptotic and survival signaling pathways in macrophage-like THP-1 cells by Shiga toxin 1. Infect Immun. 2007 Mar;75(3):1291-302. Epub 2006 Dec 28. Finally, the protein synthesis inhibitors Stx1 and anisomycin triggered limited apoptosis and prolonged JNK and p38 MAPK activation, while macrophage-like cells treated with cycloheximide remained viable and showed transient activation of MAPKs. Specific caspase inhibitors revealed that caspase-3, caspase-6, caspase-8, and caspase-9 were primarily involved in apoptosis induction. |
1(0,0,0,1) | Details |
| 15812552 | de Groot DJ, Timmer T, Spierings DC, Le TK, de Jong S, de Vries EG: Indomethacin-induced activation of the death receptor-mediated apoptosis pathway circumvents acquired doxorubicin resistance in SCLC cells. Br J Cancer. 2005 Apr 25;92(8):1459-66. Surprisingly, in GLC4-Adr indomethacin induced caspase-8 and caspase-9 activation as well as Bid cleavage, while both caspase-8 and caspase-9 specific inhibitors blocked indomethacin-induced apoptosis. Both lines were resistant to anti-Fas antibody, but plus the protein synthesis inhibitor cycloheximide anti-Fas antibody induced 40% apoptosis in GLC4-Adr. |
1(0,0,0,1) | Details |
| 18547754 | Lee K, Hyslop JM, Nanassy L, Machaty Z: Incidence of apoptosis in parthenogenetic porcine embryos generated by using protein kinase or protein synthesis inhibitors. Anim Reprod Sci. 2009 Jun;112(3-4):261-72. Epub 2008 May 3. In the present research, we investigated whether I and cycloheximide, two chemicals frequently used in combined oocyte activation protocols, have any detrimental effect on programmed cell death in the developing porcine embryo. The proportion of cells with active caspase-9 (a sign of early apoptosis) in the blastocysts produced by the different activation methods was between 19.4+/-1.9 and 23.0+/-2.4%. |
1(0,0,0,1) | Details |
| 15638359 | Polak U, Jankowska A, Warchol JB: Influence of cycloheximide on apoptosis in CHO cells, induced by ethane 1,2-dimethanesulphonate (EDS). Rocz Akad Med Bialymst. 2004;49 Suppl 1:11-3. An active form of caspase-9 and an alternation of mitochondrial transmembrane potential were also observed. |
1(0,0,0,1) | Details |
| 16446372 | Caruso JA, Mathieu PA, Joiakim A, Zhang H, Reiners JJ Jr: Aryl hydrocarbon receptor modulation of tumor necrosis factor-alpha-induced apoptosis and lysosomal disruption in a hepatoma model that is caspase-8-independent. J Biol Chem. 2006 Apr 21;281(16):10954-67. Epub 2006 Jan 30. However, Bid and pro-caspase-9, -3, and -12 processing occurred only in AhR-containing cells. |
1(0,0,0,1) | Details |
| 16585560 | Yoshioka Y, Kitao T, Kishino T, Yamamuro A, Maeda S: protects macrophages from peroxide-induced apoptosis by inducing the formation of catalase. J Immunol. 2006 Apr 15;176(8):4675-81. H2O2-treated cells showed apoptotic features, such as activation of caspase-9 and caspase-3, nuclear fragmentation, and DNA fragmentation. Cycloheximide, a protein synthesis inhibitor, inhibited both the NO-induced increase in the catalase level and the cytoprotective effect of NO. |
1(0,0,0,1) | Details |
| 18202809 | Hofmanova J, Vaculova A, Hyzd'alova M, Kozubik A: Response of normal and colon cancer epithelial cells to TNF-family apoptotic inducers. Oncol Rep. 2008 Feb;19(2):567-73. We studied apoptosis with regard to the changes at the receptor level (DR, DcR and FLIP) and at the level of mitochondria (Bid protein cleavage, Apo2.7 protein expression and caspase-9 activation). Two different approaches were used to sensitize the cells to TRAIL-induced apoptosis: inhibition of protein synthesis (cycloheximide, CHX) and inhibition of the pro-survival MEK/ERK pathway (U0126). |
1(0,0,0,1) | Details |
| 18474237 | Lee JM, Kim YJ, Ra H, Kang SJ, Han S, Koh JY, Kim YH: The involvement of caspase-11 in TPEN-induced apoptosis. FEBS Lett. 2008 Jun 11;582(13):1871-6. Epub 2008 May 12. Cycloheximide or actinomycin D blocked caspase-11 induction, reduced caspase-11 and -3 activation, and attenuated TPEN-induced neuronal apoptosis. Although mitochondria-mediated caspase-9/-3 activation also contributed to TPEN-induced apoptosis, caspase-11 is likely a key inducible apoptosis-inducing protein. |
1(0,0,0,1) | Details |
| 19136059 | Bhattacharya S, Ray RM, Johnson LR: Role of polyamines in p53-dependent apoptosis of intestinal epithelial cells. Cell Signal. 2009 Apr;21(4):509-22. Epub 2008 Dec 24. Increased p53 levels and its phosphorylation increased Bax protein, caspase-9, -3 activation and apoptosis. The translation inhibitor, cycloheximide (CHX), prevented CPT-induced apoptosis. |
1(0,0,0,1) | Details |
| 16004999 | Hareramadas B, Rai U: Mechanism of androgen-induced thymic atrophy in the wall lizard, Hemidactylus flaviviridis: an in vitro study. Gen Comp Endocrinol. 2005 Oct;144(1):10-9. DHT-induced thymocyte apoptosis was found to be caspase-dependent since it activated the initiator (caspase-9) and effector caspases (caspases-3 and -7) as well as cleaved the enzyme substrate poly (ADP- polymerase (PARP). |
1(0,0,0,1) | Details |
| 16091589 | Yui S, Saeki T, Kanamoto R, Iwami K: Characteristics of apoptosis in HCT116 colon cancer cells induced by J Biochem. 2005 Aug;138(2):151-7. Pretreatment with cycloheximide failed to inhibit apoptosis, suggesting that protein synthesis is not involved in the apoptotic response. |
0(0,0,0,0) | Details |
| 15876423 | Chow JM, Shen SC, Huan SK, Lin HY, Chen YC: but not rutin and quercitrin, prevention of H2O2-induced apoptosis via anti-oxidant activity and heme oxygenase 1 gene expression in macrophages. Biochem Pharmacol. 2005 Jun 15;69(12):1839-51. Results of Western blotting show that QE but not its glycoside rutin (RUT) and quicitrin-induced HO-1 protein expression in a time- and dose-dependent manner, and HO-1 protein induced by QE was blocked by an addition of cycloheximide or actinomycin D. |
0(0,0,0,0) | Details |
| 17879164 | Shaltouki A, Freer M, Mei Y, Weyman CM: Increased expression of the pro-apoptotic Bcl2 family member PUMA is required for mitochondrial release of cytochrome C and the apoptosis associated with skeletal myoblast differentiation. Apoptosis. 2007 Dec;12(12):2143-54. Inclusion of cycloheximide inhibits the release of cytochrome C, the activation of caspase 9 and apoptosis. |
33(0,1,1,3) | Details |
| 16478887 | Mitchell JW, Baik N, Castellino FJ, Miles LA: Plasminogen inhibits TNFalpha-induced apoptosis in monocytes. . Blood. 2006 Jun 1;107(11):4383-90. Epub 2006 Feb 14. Plasminogen treatment also markedly reduced internucleosomal DNA fragmentation and reduced levels of active caspase 3, caspase 8, and caspase 9 induced by TNFalpha or by cycloheximide. |
31(0,1,1,1) | Details |
| 17484878 | Deng W, Shuyu E, Tsukahara R, Valentine WJ, Durgam G, Gududuru V, Balazs L, Manickam V, Arsura M, VanMiddlesworth L, Johnson LR, Parrill AL, Miller DD, Tigyi G: The lysophosphatidic acid type 2 receptor is required for protection against radiation-induced intestinal injury. Gastroenterology. 2007 May;132(5):1834-51. Epub 2007 Mar 24. RESULTS: OTP was more efficacious than LPA in reducing gamma irradiation-, camptothecin-, or tumor necrosis factor alpha/cycloheximide-induced apoptosis and caspase-3-8, and caspase-9 activity in the IEC-6 cell line. |
31(0,1,1,1) | Details |
| 16966373 | Gyrd-Hansen M, Farkas T, Fehrenbacher N, Bastholm L, Hoyer-Hansen M, Elling F, Wallach D, Flavell R, Kroemer G, Nylandsted J, Jaattela M: Apoptosome-independent activation of the lysosomal cell death pathway by caspase-9. Mol Cell Biol. 2006 Nov;26(21):7880-91. Epub 2006 Sep 11. |
5(0,0,0,5) | Details |
| 16260615 | Ohgushi M, Kuroki S, Fukamachi H, O'Reilly LA, Kuida K, Strasser A, Yonehara S: Transforming growth factor beta-dependent sequential activation of Smad, Bim, and caspase-9 mediates physiological apoptosis in gastric epithelial cells. Mol Cell Biol. 2005 Nov;25(22):10017-28. |
3(0,0,0,3) | Details |
| 15754039 | Lai MD, Lin WC, Sun YM, Chang FL: Phosphorylated and hypoacetylated mutant p53 enhances cisplatin-induced apoptosis through caspase-9 pathway in the absence of transcriptional activation or translation. Int J Mol Med. 2005 Apr;15(4):725-34. Both transcriptional inhibitor actinomycin D and translational inhibitor cycloheximide did not inhibit apoptosis. |
3(0,0,0,3) | Details |
| 15863130 | Hougardy BM, van der Zee AG, van den Heuvel FA, Timmer T, de Vries EG, de Jong S: Sensitivity to Fas-mediated apoptosis in high-risk HPV-positive human cervical cancer cells: relationship with Fas, caspase-8, and Bid. Gynecol Oncol. 2005 May;97(2):353-64. Analysis of the Fas apoptotic pathway showed that anti-Fas treatment induced caspase-8 activation and concomitantly Bid cleavage, caspase-9 and caspase-3 activation, PARP cleavage and apoptosis in HeLa and CaSki. |
3(0,0,0,3) | Details |
| 16109713 | Rahmani M, Davis EM, Bauer C, Dent P, Grant S: Apoptosis induced by the kinase inhibitor BAY 43-9006 in human leukemia cells involves down-regulation of Mcl-1 through inhibition of translation. J Biol Chem. 2005 Oct 21;280(42):35217-27. Epub 2005 Aug 18. Here we report that treatment with BAY 43-9006 results in marked cytochrome c and AIF release into the cytosol, caspase-9, -8, -7, and -3 activation, and apoptosis in human leukemia cells (U937, Jurkat, and K562). Inhibition of protein synthesis by cycloheximide or proteasome function with MG132 and pulse-chase studies with [35S] demonstrated that BAY 43-9006 did not diminish Mcl-1 protein stability, nor did it enhance Mcl-1 ubiquitination, but instead markedly attenuated Mcl-1 translation in association with the rapid and potent dephosphorylation of the eIF4E translation initiation factor. |
2(0,0,0,2) | Details |
| 18078929 | Brumatti G, Yon M, Castro FA, Bueno-da-Silva AE, Jacysyn JF, Brunner T, Amarante-Mendes GP: Conversion of CD95 (Fas) Type II into Type I signaling by sub-lethal doses of cycloheximide. Exp Cell Res. 2008 Feb 1;314(3):554-63. Epub 2007 Nov 17. The other is related to the cleavage of Bid by low concentration of caspase-8, leading to the release of cytochrome c from mitochondria and the activation of caspase-3 by the cytochrome c/APAF-1/caspase-9 apoptosome (Type II cells). |
1(0,0,0,1) | Details |
| 15685617 | Suy S, Mitchell JB, Samuni A, Mueller S, Kasid U: tempo, a small molecule, induces apoptosis in prostate carcinoma cells and suppresses tumor growth in athymic mice. Cancer. 2005 Mar 15;103(6):1302-13. Tempo-induced loss of cell viability was blocked partially or completely after pretreatment of cells with actinomycin-D or cycloheximide, suggesting a de novo macromolecule synthesis-dependent mechanism of cell death. Enzymatic assays were performed to measure the activities of 2 proteases, i.e., caspase-9 and caspase-3, in tempo-treated cells. |
2(0,0,0,2) | Details |
| 18977202 | Nakashima T, Tanaka R, Yamashita Y, Kanda Y, Hara M: Aranorosin and a novel derivative inhibit the anti-apoptotic functions regulated by Bcl-2. Biochem Biophys Res Commun. 2008 Dec 26;377(4):1085-90. Epub 2008 Oct 31. Furthermore, K050 inhibited anti-apoptotic functions regulated by Bcl-2, resulting in a Fas-triggered mitochondrial transmembrane potential loss, the activation of caspase-9, and a morphological change to apoptosis. |
1(0,0,0,1) | Details |