| Name | osteocalcin |
|---|---|
| Synonyms | BGLAP; BGP; Bone Gla protein; Bone gamma carboxyglutamaic acid protein; Gamma carboxyglutamicacid protein; Gamma carboxyglutamic acid containing protein; Osteocalcin; Osteocalcin precursor… |
| Name | cycloheximide |
|---|---|
| CAS |
| PubMed | Abstract | RScore(About this table) | |
|---|---|---|---|
| 17203194 | Uchiyama S, Yamaguchi M: and zinc synergistically enhance gene expression and mineralization in osteoblastic MC3T3-E1 cells. Int J Mol Med. 2007 Feb;19(2):213-20. The effect of (10 (-5) M) plus zinc (10 (-4) M) in enhancing the expression of alpha1 (I) collagen, alkaline phosphatase, or osteocalcin mRNAs in osteoblastic cells was completely prevented with a culture of cycloheximide (10 (-7) M), an inhibitor of protein synthesis, or 5,6-dichloro-1-beta-D-ribofuranosylbenimidazole (DRB) (10 (-6) M), an inhibitor of transcriptional activity. |
33(0,1,1,3) | Details |
| 17113042 | Hsu YL, Chang JK, Tsai CH, Chien TT, Kuo PL: induces human osteoblast differentiation through bone morphogenetic protein-2/p38 mitogen-activated protein kinase pathway. Biochem Pharmacol. 2007 Feb 15;73(4):504-14. Epub 2006 Oct 26. By means of alkaline phosphatase (ALP) activity, osteocalcin, and type I collagen enzyme-linked immunosorbent assay (ELISA), we have shown that exhibits a significant induction of differentiation in MG-63 and hFOB human osteoblasts. |
4(0,0,0,4) | Details |
| 17368935 | Choi EM: Modulatory effects of on osteoblastic function and inflammatory mediators in osteoblastic MC3T3-E1 cells. Cell Biol Int. 2007 Sep;31(9):870-7. Epub 2007 Feb 11. (1microM) caused a significant elevation of collagen content, alkaline phosphatase (ALP) activity, and osteocalcin secretion in the cells (P <0.05). The effect of in increasing collagen content and ALP activity was completely prevented by the presence of 10 (-6) M cycloheximide and 10 (-6) M tamoxifen, suggesting that luteolin's effect results from a newly synthesized protein component and might be partly involved in action. |
1(0,0,0,1) | Details |
| 16350840 | Kirimoto A, Takagi Y, Ohya K, Shimokawa H: Effects of on the differentiation of chondrogenic progenitor cells, ATDC5. J Med Dent Sci. 2005 Sep;52(3):153-62. The addition of protein synthesis inhibitor, cycloheximide (CHX), partially inhibits the induction of type X collagen and MMP-13 mRNA by RA. However, RA downregulates ALPase, bone gla protein (BGP) mRNAs and mineralization. |
1(0,0,0,1) | Details |
| 16204952 | Lee KH, Choi EM: stimulates osteoblastic differentiation and inhibits peroxide-induced production of inflammatory mediators in MC3T3-E1 cells. Biol Pharm Bull. 2005 Oct;28(10):1948-53. (1-50 microM) caused a significant elevation of cell growth, alkaline phosphatase (ALP) activity, collagen content, and osteocalcin secretion in osteoblastic MC3T3-E1 cells (p <0.05). The effect of (10 microM) in increasing ALP activity and collagen content was completely prevented by the presence of 10 (-6) M cycloheximide and 10 (-6) M tamoxifen, suggesting that A's effect results from a newly synthesized protein component and might be partly involved in action. |
1(0,0,0,1) | Details |
| 18327666 | Yamaguchi M, Goto M, Uchiyama S, Nakagawa T: Effect of zinc on gene expression in osteoblastic MC3T3-E1 cells: enhancement of Runx2, OPG, and regucalcin mRNA expressions. Mol Cell Biochem. 2008 May;312(1-2):157-66. Epub 2008 Mar 9. The effect of zinc on the mRNA expressions in Runx2, osteocalcin, alpha1 (I) collagen, insulin-like growth factor-I (IGF-I), transforming growth factor-beta1 (TGF-beta1), osteoprotegerin (OPG), regucalcin, zinc transporter 1 (ZIP1), or glyceroaldehyde-3- dehydrogenase (G3PDH) in osteoblastic MC3T3-E1 cells in vitro was investigated. The stimulatory effects of zinc on Runx2, OPG, or regucalcin mRNAs were significantly prevented in the presence of cycloheximide (10 (-7) M), an inhibitor of protein synthesis, or 5,6-dichloro-1-beta-D-ribofuranosyl-benzimidazole (10 (-6) M), an inhibitor of transcription activity. |
1(0,0,0,1) | Details |
| 15922308 | Choi EM: The licorice root derived isoflavan glabridin increases the function of osteoblastic MC3T3-E1 cells. Biochem Pharmacol. 2005 Aug 1;70(3):363-8. Glabridin (1-10microM) significantly increased the growth of MC3T3-E1 cells and caused a significant elevation of alkaline phosphatase (ALP) activity, collagen content and osteocalcin secretion in the cells (P <0.05). The effect of glabridin (10microM) in increasing ALP activity and collagen content was completely prevented by the presence of 10 (-6) M cycloheximide and 10 (-6) M tamoxifen, suggesting that glabridin's effect results from a newly synthesized protein component and might be partly involved in action. |
1(0,0,0,1) | Details |
| 16883635 | Lee KH, Choi EM: Rubus coreanus Miq. extract promotes osteoblast differentiation and inhibits bone-resorbing mediators in MC3T3-E1 cells. Am J Chin Med. 2006;34(4):643-54. RCE (10approximately50 microg/ml) caused a significant elevation in cell viability, alkaline phosphatase (ALP) activity, collagen content, and osteocalcin secretion in the cells. The effect of RCE (50 microg/ml) in increasing cell viability, ALP activity, and collagen content was prevented by the presence of 10 (-6) M cycloheximide and 10 (-6) M tamoxifen, suggesting that RCE's effect results from a newly synthesized protein component and might be partly involved in action. |
1(0,0,0,1) | Details |
| 17899321 | Uchiyama S, Yamaguchi M: Anabolic effect of in osteoblastic MC3T3-E1 cells is enhanced with 17beta- or zinc in vitro: the unique effect with zinc on Runx2 and alpha1 (I) collagen mRNA expressions. Mol Cell Biochem. 2008 Jan;307(1-2):209-19. Epub 2007 Sep 25. Such an effect was not seen in the presence of cycloheximide (10 (-7) M), an inhibitor of protein synthesis, or 5,6-dichloro-1-beta-D: -ribofuranosyl-benzimidazole (DRB; 10 (-6) M), an inhibitor of transcriptional activity. |
0(0,0,0,0) | Details |
| 16949358 | Ito S, Suzuki N, Kato S, Takahashi T, Takagi M: Glucocorticoids induce the differentiation of a mesenchymal progenitor cell line, ROB-C26 into adipocytes and osteoblasts, but fail to induce terminal osteoblast differentiation. Bone. 2007 Jan;40(1):84-92. Epub 2006 Sep 1. To clarify the effects of glucocorticoids (GCs) on osteoblast and adipocyte differentiation, we investigated the effects of dexamethasone (Dex), a GC analogue on transcription factors for osteoblasts (Runx2, Dlx5 and Osterix) and adipocytes (C/EBPs such as C/EBPalpha, C/EBPbeta and C/EBPdelta, and PPARgamma2), late osteoblastic markers, bone sialoprotein (BSP) and osteocalcin (OC), and adipocyte differentiation-dependent protein, aP2 in a clonal mesenchymal progenitor cell line, ROB-C26 (C26). Cycloheximide pretreatment indicated that Dex signaling immediately increases expressions of the C/EBPs and Dlx5, while expressions of the rest of the genes require de novo protein synthesis. |
1(0,0,0,1) | Details |