| Name | interleukin 8 |
|---|---|
| Synonyms | 3 10C; AMCF I; CXC chemokine ligand 8; CXCL 8; CXCL8; Emoctakin; GCP 1; GCP1… |
| Name | cycloheximide |
|---|---|
| CAS |
| PubMed | Abstract | RScore(About this table) | |
|---|---|---|---|
| 16085794 | Huang HC, Tai FY, Wang FS, Liu CA, Hsu TY, Ou CY, Yang KD: Correlation of augmented IL-8 production to premature chronic lung disease: implication of posttranscriptional regulation. Pediatr Res. 2005 Aug;58(2):216-21. Epub 2005 Jul 29. The augmented IL-8 mRNA expression in cord blood was inhibited by actinomycin D but enhanced by cycloheximide, suggesting that IL-8 production is controlled by de novo transcriptional induction as well as posttranscriptional up-regulation of IL-8 by neonatal leukocytes, relating to the development of CLD. |
85(1,1,1,5) | Details |
| 16197996 | Sato H, Miura R, Kai C: Measles virus infection induces interleukin-8 release in human pulmonary epithelial cells. Comp Immunol Microbiol Infect Dis. 2005 Jul;28(4):311-20. Epub 2005 Sep 28. The IL-8 production was little affected by UV-inactivation of MV and scarcely suppressed by cycloheximide treatment. |
85(1,1,1,5) | Details |
| 17286758 | Endoh I, Di Girolamo N, Hampartzoumian T, Cameron B, Geczy CL, Tedla N: Ultraviolet B irradiation selectively increases the production of interleukin-8 in human cord blood-derived mast cells. Clin Exp Immunol. 2007 Apr;148(1):161-7. Cycloheximide reduced the UVB-mediated induction of IL-8 by 30-40%, suggesting that new protein synthesis contributed to IL-8 production. |
84(1,1,1,4) | Details |
| 17671691 | Kamohara H, Takahashi M, Ishiko T, Ogawa M, Baba H: Induction of interleukin-8 (CXCL-8) by tumor necrosis factor-alpha and leukemia inhibitory factor in pancreatic carcinoma cells: Impact of CXCL-8 as an autocrine growth factor. Int J Oncol. 2007 Sep;31(3):627-32. Actinomycin D suppressed and cycloheximide augmented CXCL-8 mRNA which was induced by TNF-alpha or not. |
40(0,1,1,10) | Details |
| 16479515 | Green J, Khabar KS, Koo BC, Williams BR, Polyak SJ: Stability of CXCL-8 and related AU-rich mRNAs in the context of hepatitis C virus replication in vitro. J Infect Dis. 2006 Mar 15;193(6):802-11. Epub 2006 Feb 13. CXCL-8 mRNA was superinduced by TNF- alpha in the presence of the protein-synthesis inhibitor cycloheximide. |
39(0,1,1,9) | Details |
| 16541418 | Lu S, Dong Z: Characterization of TGF-beta-regulated interleukin-8 expression in human prostate cancer cells. Prostate. 2006 Jun 15;66(9):996-1004. TGF-beta1 stimulated IL-8 expression in dose- and time-dependent manners, which was blocked by cycloheximide (CHX) and actinomycin D (ActD). |
38(0,1,1,8) | Details |
| 19699525 | Harashima A, Toraya T, Okochi A, Yamamoto M, Suzuki M, Otani T, Inoue T, Tsuji-Takayama K, Sugimoto A, Takeuchi M, Yamasaki F, Nakamura S, Kibata M: Interleukin-8 and RANTES are signature cytokines made by HOZOT, a new type of regulatory T cells. Mol Immunol. 2009 Oct;46(16):3310-9. Epub 2009 Aug 21. Interestingly, IL-8 mRNA was induced by cycloheximide treatment and RANTES showed reduced mRNA but increased protein expression by antibody stimulation. |
36(0,1,1,6) | Details |
| 17606477 | MacManus CF, Pettigrew J, Seaton A, Wilson C, Maxwell PJ, Berlingeri S, Purcell C, McGurk M, Johnston PG, Waugh DJ: Interleukin-8 signaling promotes translational regulation of cyclin D in androgen-independent prostate cancer cells. Mol Cancer Res. 2007 Jul;5(7):737-48. Epub 2007 Jul 2. Administration of recombinant human IL-8 induced a rapid, time-dependent increase in cyclin D1 expression in AIPC cells, a response attenuated by the translation inhibitor cycloheximide but not by the RNA synthesis inhibitor, actinomycin D. |
8(0,0,0,8) | Details |
| 16434099 | Gonzalez TV, Farrant SA, Mantis NJ: Ricin induces IL-8 secretion from human monocyte/macrophages by activating the p38 MAP kinase pathway. Mol Immunol. 2006 Apr;43(11):1920-3. Epub 2006 Jan 24. |
6(0,0,0,6) | Details |
| 16714014 | Mormina ME, Thakur S, Molleman A, Whelan CJ, Baydoun AR: Cannabinoid signalling in TNF-alpha induced IL-8 release. Eur J Pharmacol. 2006 Jul 1;540(1-3):183-90. Epub 2006 May 5. |
5(0,0,0,5) | Details |
| 19648110 | King EM, Holden NS, Gong W, Rider CF, Newton R: Inhibition of NF-kappaB-dependent transcription by MKP-1: transcriptional repression by glucocorticoids occurring via p38 MAPK. J Biol Chem. 2009 Sep 25;284(39):26803-15. Epub 2009 Jul 31. Since NF-kappaB-dependent transcription and IL-8 protein, mRNA, and unspliced RNA (a surrogate of transcription rate) are sensitive to p38 MAPK inhibitors (SB203580 and SB239063), we explored the role of MKP-1 in repression of these outputs. |
4(0,0,0,4) | Details |
| 17064399 | Kubota K, Sakaki H, Imaizumi T, Nakagawa H, Kusumi A, Kobayashi W, Satoh K, Kimura H: -inducible gene-I is induced in gingival fibroblasts by lipopolysaccharide or poly IC: possible roles in interleukin-1beta, -6 and -8 expression. Oral Microbiol Immunol. 2006 Dec;21(6):399-406. We also addressed the role of RIG-I in the expression of IL-1beta, IL-6 and IL-8 in gingival fibroblasts stimulated with LPS or dsRNA. The effect of cycloheximide, a protein synthesis inhibitor, on RIG-I induction by these stimuli was examined. |
4(0,0,0,4) | Details |
| 16157236 | Su SJ, Huang LW, Pai LS, Liu HW, Chang KL: at pathophysiologic concentrations activates human monocyte and induces cytokine expression and inhibits macrophage migration inhibitory factor expression. Nutrition. 2005 Oct;21(10):994-1002. METHODS: Human monocytes were treated in vitro with a variety of concentrations that ranged from physiologic concentration to higher than pathophysiologic concentration, and we analyzed their expressions of inflammatory cytokines, including tumor necrosis factor-alpha, interleukin-1beta, interleukin-6, interleukin-8, interleukin-12, and migration inhibitory factor. |
2(0,0,0,2) | Details |
| 16087364 | Relova AJ, Shahana S, Makeeva N, Roomans GM: Effect of cytokines on ICAM-1 and ZO-1 expression on human airway epithelial cells. Cell Biol Int. 2005 Sep;29(9):768-77. The study aimed at investigating the effects of interleukin (IL)-4, IL-8, IL-13 and interferon-gamma (IFN-gamma) on cell viability and intracellular adhesion molecule (ICAM)-1 and zonula occludens protein (ZO)-1 expression on cultured human basal and columnar airway epithelial cells. Cycloheximide (CHX) induced cell death in both cell lines. |
2(0,0,0,2) | Details |
| 16606835 | Uzonyi B, Lotzer K, Jahn S, Kramer C, Hildner M, Bretschneider E, Radke D, Beer M, Vollandt R, Evans JF, Funk CD, Habenicht AJ: Cysteinyl leukotriene 2 receptor and protease-activated receptor 1 activate strongly correlated early genes in human endothelial cells. Proc Natl Acad Sci U S A. 2006 Apr 18;103(16):6326-31. Epub 2006 Apr 10. Transcripts peaked at approximately 60 min, were unaffected by a cysLT1-R antagonist, and were superinduced by cycloheximide. Strongly induced genes were studied in detail: Early growth response (EGR) and nuclear receptor subfamily 4 group A transcription factors; E-selectin; CXC ligand 2; IL-8; a disintegrin-like and metalloprotease (reprolysin type) with thrombospondin type 1 motif 1 (ADAMTS1); Down syndrome critical region gene 1 (DSCR1); tissue factor (TF); and cyclooxygenase 2. |
2(0,0,0,2) | Details |
| 17948269 | Rossato M, Cencig S, Gasperini S, Cassatella MA, Bazzoni F: IL-10 modulates cytokine gene transcription by protein synthesis-independent and dependent mechanisms in lipopolysaccharide-treated neutrophils. Eur J Immunol. 2007 Nov;37(11):3176-89. Here, we demonstrate that, in neutrophils "preconditioned" by LPS, IL-10 primarily targets the transcription of TNF-alpha, CXCL8 and IL-1ra genes, as revealed by primary transcript real-time RT-PCR. Importantly, inhibition of IL-10-induced STAT3 activation and IL-10-suppressive action by a prolonged exposure to cycloheximide (CHX) was observed to occur also in human monocytes and was caused by a defective IL-10-mediated activation of Jak1 and Tyk2 kinases. |
2(0,0,0,2) | Details |
| 17304101 | Pearl-Yafe M, Fabian I, Halperin D, Flatau E, Werber S, Shalit I: Interferon-gamma and bacterial lipopolysaccharide act synergistically on human neutrophils enhancing interleukin-8, interleukin-1beta, tumor necrosis factor-alpha, and interleukin-12 p70 secretion and phagocytosis via upregulation of toll-like receptor 4. Shock. 2007 Mar;27(3):226-31. Using the protein synthesis inhibitor cycloheximide and measuring the expression of CD35 in neutrophils stimulated with IFN-gamma and LPS alone or in combination, we could demonstrate that IFN-gamma enhances TLR4 by de novo protein synthesis, whereas the addition of LPS acts synergistically by enhancing vesicular mobilization to the cell surface. |
2(0,0,0,2) | Details |
| 16826536 | Chan G, Guilbert LJ: Ultraviolet-inactivated human cytomegalovirus induces placental syncytiotrophoblast apoptosis in a Toll-like receptor-2 and tumour necrosis factor-alpha dependent manner. J Pathol. 2006 Sep;210(1):111-20. ST cultures challenged with UV-HCMV increased transcription and secretion of the inflammatory cytokines tumour necrosis factor alpha (TNFalpha) and interleukin-8, and antibody to TNFalpha inhibited UV-HCMV-induced apoptosis. Treatment with cycloheximide, an inhibitor of protein translation, did not reduce UV-HCMV-induced TNFalpha gene transcription, indicating that upregulation was independent of de novo protein synthesis. |
1(0,0,0,1) | Details |
| 19248116 | Yammani RR, Long D, Loeser RF: Interleukin-7 stimulates secretion of S100A4 by activating the JAK/STAT signaling pathway in human articular chondrocytes. Arthritis Rheum. 2009 Mar;60(3):792-800. METHODS: Human articular chondrocytes isolated from ankle cartilage were stimulated with 10 ng/ml of interleukin-1beta (IL-1beta), IL-6, IL-7, or IL-8. Cells were pretreated with either a JAK-3 inhibitor, brefeldin A, or cycloheximide. |
1(0,0,0,1) | Details |
| 17405813 | Neel NF, Lapierre LA, Goldenring JR, Richmond A: RhoB plays an essential role in CXCR2 sorting decisions. J Cell Sci. 2007 May 1;120(Pt 9):1559-71. Epub 2007 Apr 3. Expression of RhoB T19N and transfection of RhoB siRNA impaired sorting of CXCR2 to the lysosome after 3 hours of CXCL8 stimulation and impaired CXCL8-induced CXCR2 degradation. |
2(0,0,0,2) | Details |
| 19446037 | Safronova O, Pluemsampant S, Nakahama K, Morita I: Regulation of chemokine gene expression by hypoxia via cooperative activation of NF-kappaB and histone deacetylase. Int J Biochem Cell Biol. 2009 Nov;41(11):2270-80. Epub 2009 May 13. We found that despite the similarities of the promoter structures of IL-8 and MCP-1, these chemokines were differently regulated by hypoxia (an increase in IL-8, but a decrease in MCP-1 mRNA and protein expression). |
2(0,0,0,2) | Details |
| 18729741 | Shizu M, Itoh Y, Sunahara R, Chujo S, Hayashi H, Ide Y, Takii T, Koshiko M, Chung SW, Hayakawa K, Miyazawa K, Hirose K, Onozaki K: Cigarette smoke condensate upregulates the gene and protein expression of proinflammatory cytokines in human fibroblast-like synoviocyte line. J Interferon Cytokine Res. 2008 Aug;28(8):509-21. When human fibroblast-like synoviocytes line MH7A was treated with cigarette smoke condensate (CSC), either mainstream or sidestream, expression levels of interleukin (IL)-1alpha, IL-1beta, IL-6, IL-8, and CYP1A1 mRNA were upregulated in both time- and dose-dependent manners. Cycloheximide treatment indicated that the augmenting effect of CSC on IL-1alpha, IL-1beta and IL-8, but not IL-6 and CYP1A1, mRNA expression requires de novo protein synthesis. |
1(0,0,0,1) | Details |
| 19439980 | Triggiani M, Granata F, Petraroli A, Loffredo S, Frattini A, Staiano RI, Monaco G, Marone G: Inhibition of secretory phospholipase A2-induced cytokine production in human lung macrophages by budesonide. Int Arch Allergy Immunol. 2009;150(2):144-55. Epub 2009 May 11. At the end of incubation the release of TNF-alpha, IL-6 and IL-8 was assessed by ELISA. |
2(0,0,0,2) | Details |
| 16344041 | Choi JJ, Jin M, Lee JK, Lee WY, Park YI, Han YN, Kim S: Control of cytokine gene expression by PG101, a water-soluble extract prepared from Lentinus lepideus. Biochem Biophys Res Commun. 2006 Jan 20;339(3):880-7. The RNA level of TNF-alpha, MIP-1alpha, MIP-1beta, MIP-3alpha, and IL-8 was not affected by the presence of cycloheximide, an inhibitor of the translation process, suggesting that they are the direct targets of PG101. A significantly high protein level of TNF-alpha, MIP-1alpha, and IL-8 remained detectable, even when cells were cultured with actinomycin D, 2h prior to the PG101 treatment. |
1(0,0,0,1) | Details |
| 15582587 | Bischoff DS, Zhu JH, Makhijani NS, Yamaguchi DT: KC chemokine expression by TGF-beta in C3H10T1/2 cells induced towards osteoblasts. Biochem Biophys Res Commun. 2005 Jan 14;326(2):364-70. KC is a member of the CXC chemokine family with homology to human IL-8, a potent neutrophilic chemotactic cytokine. Use of the translational inhibitor cycloheximide results in mRNA "superinduction" suggesting other factors are involved that normally function to down-regulate KC expression. |
1(0,0,0,1) | Details |
| 16603617 | Mahdy AM, Lowes DA, Galley HF, Bruce JE, Webster NR: Production of soluble triggering receptor expressed on myeloid cells by lipopolysaccharide-stimulated human neutrophils involves de novo protein synthesis. Clin Vaccine Immunol. 2006 Apr;13(4):492-5. Activation of the receptor induces neutrophils to release the enzyme myeloperoxidase and inflammatory cytokines such as interleukin-8. However, sTREM-1 release from neutrophils was significantly upregulated by LPS stimulation (P < 0.0001), an effect that was abrogated by cycloheximide. |
1(0,0,0,1) | Details |
| 16549420 | Na YJ, Yang SH, Baek DW, Lee DH, Kim KH, Choi YM, Oh ST, Hong YS, Kwak JY, Lee KS: Effects of peritoneal fluid from endometriosis patients on the release of vascular endothelial growth factor by neutrophils and monocytes. Hum Reprod. 2006 Jul;21(7):1846-55. Epub 2006 Mar 20. The VEGF mRNA expression levels in the neutrophils were higher in the EPF, which was abrogated by cycloheximide, suggesting that the EPF induces the production of VEGF in neutrophils. |
0(0,0,0,0) | Details |