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Cascio M, Schoppa NE, Grodzicki RL, Sigworth FJ, Fox RO: Functional expression and purification of a homomeric human alpha 1 glycine receptor in baculovirus-infected insect cells. J Biol Chem. 1993 Oct 15;268(29):22135-42.
These channels were gated by glycine, but not in the presence of strychnine. An immunoreactive 48-kDa protein could be easily visualized on Coomassie-stained sodium dodecyl sulfate-polyacrylamide gels of whole-cell lysates with maximal expression 3 days postinfection. |
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