| 9870526 |
Parandoosh Z, Bogowitz CA, Nova MP: A fluorometric assay for the measurement of endothelial cell density in vitro. In Vitro Cell Dev Biol Anim. 1998 Nov-Dec;34(10):772-6.
In preliminary studies, three substrates--p-nitrophenyl phosphate, 4-methylumbelliferyl phosphate, and 2'-[2-benzthiazoyl]-6'-hydroxy-benthiazole phosphate (AttoPhos)--were compared with respect to their kinetic, optimum assay conditions, sensitivity, and detection limits.
In subsequent experiments, assay conditions were validated for measuring endothelial cell density in response to basic fibroblast growth factor and fumagillin. |
1(0,0,0,1) |
Details |