Protein Information

Name acetolactate synthase
Synonyms Acetolactate synthase; OR10B1P

Compound Information

Name imazapyr
CAS

Reference List

PubMed Abstract RScore(About this table)
16665871 Muhitch MJ: Acetolactate Synthase Activity in Developing Maize (Zea mays L.) Kernels. Plant Physiol. 1988 Jan;86(1):23-27.

When assayed using unpurified homogenates, embryo acetolactate synthase activity appeared less sensitive to inhibition by leucine + valine and by the imidazolinone herbicide imazapyr than endosperm acetolactate synthase activity.
35(0,1,1,5) Details
16653216 Christopher JT, Powles SB, Holtum JA: Resistance to Acetolactate Synthase-Inhibiting Herbicides in Annual Ryegrass (Lolium rigidum) Involves at Least Two Mechanisms. Plant Physiol. 1992 Dec;100(4):1909-1913.

The concentrations of herbicide required to reduce in vitro acetolactate synthase (ALs) activity 50% with respect to control assays minus herbicide for biotype WLR1 was greater than those for susceptible biotype VLR1 by a factor of > 30, > 30, 7,4, and 2 for the herbicides chlorsulfuron, sulfometuron-methyl, imazapyr, imazathapyr, and imazamethabenz, respectively.
32(0,1,1,2) Details
12476991 Aviv D, Amsellem Z, Gressel J: Transformation of carrots with mutant acetolactate synthase for Orobanche (broomrape) control. Pest Manag Sci. 2002 Dec;58(12):1187-93.

We report the successful engineering of a mutant acetolactate synthase (ALS) gene into carrot, allowing control of broomrape already in heterozygotes of the first back-crossed generation, by imazapyr, an imidazolinone ALS inhibitor.
33(0,1,1,3) Details
19280593 Gressel J: Crops with target-site herbicide resistance for Orobanche and Striga control. Pest Manag Sci. 2009 May;65(5):560-5.

Such target-site resistances have allowed foliar applications of herbicides inhibiting enol-pyruvylshikimate phosphate synthase (EPSPS) (glyphosate), acetolactate synthase (ALS) (e.g. chlorsulfuron, imazapyr) and dihydropteroate synthase (asulam) for Orobanche control in experimental conditions with various crops.
31(0,1,1,1) Details
7767237 Mourad G, Williams D, King J: A double mutant allele, csr1-4, of Arabidopsis thaliana encodes an acetolactate synthase with altered kinetics. Planta. 1995;196(1):64-8.

The mutants used were the chlorsulfuron-resistant GH50 (csr1-1), the imazapyr-resistant GH90 (csr1-2), the triazolopyrimidine-resistant Tzp5 (csr1-3) and the multiherbicide-resistant, double mutant GM4.8 (csr1-4), derived from csr1-1 and csr1-2 by intragenic recombination (G.
3(0,0,0,3) Details
16418231 Endo M, Osakabe K, Ichikawa H, Toki S: Molecular characterization of true and ectopic gene targeting events at the acetolactate synthase gene in Arabidopsis. Plant Cell Physiol. 2006 Mar;47(3):372-9. Epub 2006 Jan 17.

To confirm the generality of the GT system in Arabidopsis, and to characterize the EGT event in plants in detail, we converted ALS from a herbicide (imazapyr)-susceptible to a -resistant form by GT.
2(0,0,0,2) Details
11813100 Preston C, Powles SB: Evolution of herbicide resistance in weeds: initial frequency of target site-based resistance to acetolactate synthase-inhibiting herbicides in Lolium rigidum. Heredity. 2002 Jan;88(1):8-13.

The frequency of individuals resistant to the sulfonylurea herbicide sulfometuron-methyl varied from 2.2 x 10 (-5) to 1.2 x 10 (-4) and the frequency of individuals resistant to the imidazolinone herbicide imazapyr varied from 1 x 10 (-5) to 5.8 x 10 (-5) depending on the population.
2(0,0,0,2) Details
16667804 Saxena PK, King J: Lack of Cross-Resistance of Imidazolinone-Resistant Cell Lines of Datura innoxia P. Plant Physiol. 1990 Nov;94(3):1111-1115.

Two cell lines of Datura innoxia resistant to two imidazolinone herbicides, imazapyr and imazaquin, were isolated from mutagenized, predominantly haploid cell suspension cultures.
The variation in resistance and cross-resistance of chlorsulfuron-resistant (PK Saxena, J King [1988] Plant Physiol 86: 863-867) and imidazolinone-resistant cell lines of Datura innoxia demonstrates the possibility of separate mutations of acetolactate synthase gene resulting in specific phenotypes.
1(0,0,0,1) Details
16668488 Sathasivan K, Haughn GW, Murai N: Molecular Basis of Imidazolinone Herbicide Resistance in Arabidopsis thaliana var Columbia. Plant Physiol. 1991 Nov;97(3):1044-1050.

Acetolactate synthase (ALS), the first enzyme in the biosynthetic pathway of leucine, isoleucine, and valine, is inhibited by imidazolinone herbicides.
To understand the molecular basis of imidazolinone resistance, we isolated the ALS gene from an imazapyr-resistant mutant GH90 of Arabidopsis thaliana.
1(0,0,0,1) Details
14620047 Osuna MD, Fischer AJ, De Prado R: Herbicide resistance in Aster squamatus conferred by a less sensitive form of acetolactate synthase. Pest Manag Sci. 2003 Nov;59(11):1210-6.

A biotype of Aster squamatus (Sprengel) Hieronymus with suspected resistance to the ALS-inhibiting herbicide imazapyr was detected in a chicken farm in the province of Seville, Spain, which had been treated once a year with imazapyr for 10 years.
1(0,0,0,1) Details
12974347 Kuk YI, Jung HI, Kwon OD, Lee DJ, Burgos NR, Guh JO: Sulfonylurea herbicide-resistant Monochoria vaginalis in Korean rice culture. Pest Manag Sci. 2003 Sep;59(9):949-61.

In vitro acetolactate synthase (ALS) activity of the R biotype was 183, 35, 130 and 31 times more resistant to imazosulfuron, bensulfuron-methyl, cyclosulfamuron and pyrazosulfuron-ethyl, respectively, than the S biotype.
Accession #9 exhibited cross-resistance to other sulfonylurea herbicides, bensulfuron-methyl, cyclosulfamuron and pyrazosulfuron-ethyl, but not to the imidazolinone herbicides, imazapyr and imazaquin.
1(0,0,0,1) Details