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Takeda Y, Ogiso Y, Miwatani T: Effect of zinc ion on the hemolytic activity of thermostable direct hemolysin from Vibrio parahaemolyticus, streptolysin O, and Triton X-100. Infect Immun. 1977 Aug;17(2):239-43.
We found that in certain buffers, such as tris (hydroxymethyl)-aminomethane-hydrochloride, boric acid-borax, and N-hydroxyethyl piperazine-N'-2-ethanesulfonic acid-sodium hydroxide, hemoglobins released from erythrocytes were easily precipitated by addition of Zn2+, thus resulting in a false inhibition of hemolysin by Zn2+ when hemolysis was assayed by measuring absorbance at 540 nm of released hemoglobins. |
83(1,1,1,3) |
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