Protein Information

Name protein a
Synonyms A1 activator; PSAP; Protein C; CSAct; Cerebroside sulfatase activator; Cerebroside sulfate activator; Co beta glucosidase; Component C…

Compound Information

Name atrazine
CAS

Reference List

PubMed Abstract RScore(About this table)
10552824 Ohki A, Kuboyama N, Koizumi K, Tanaka A, Sato Y, Kohno H, Boger P, Wakabayashi K: Mode of action of novel 2-(benzylamino)-4-methyl-6-(trifluoro- methyl)-1,3,5-triazine herbicides: inhibition of photosynthetic electron transport and binding studies. J Agric Food Chem. 1999 Oct;47(10):4398-402.

The replacement of the bound [(14) C] atrazine by 1,3,5-triazines was tested to determine whether the novel 1,3,5-triazine analogues exhibit the same binding pattern at the D1-protein as atrazine.
81(1,1,1,1) Details
16228382 Ikeda Y, Ohki S, Koizumi K, Tanaka A, Watanabe H, Kohno H, van Rensen JJ, Boger P, Wakabayashi K: Binding site of novel 2-benzylamino-4-methyl-6-trifluoromethyl-1,3,5-triazine herbicides in the D1 protein of Photosystem II. Photosynth Res. 2003;77(1):35-43.

Thus, 2-benzylamino-4-methyl-6-trifluoromethyl-1,3,5-triazine herbicides are considered to bind to the same niche at the D1 protein as atrazine, but use amino acid residue (s) different from those involved with atrazine binding.
31(0,1,1,1) Details
8976049 Steegborn C, Skladal P: Construction and characterization of the direct piezoelectric immunosensor for atrazine operating in solution. Biosens Bioelectron. 1997;12(1):19-27.

The interaction of the anti atrazine monoclonal antibody (MAb, clone D6F3) with the immobilized atrazine was characterized using both crude ascitic fluid and Protein A-purified MAb preparates.
31(0,1,1,1) Details
1515117 Guilbault GG, Hock B, Schmid R: A piezoelectric immunobiosensor for atrazine in drinking water. Biosens Bioelectron. 1992;7(6):411-9.

Atrazine antibodies (polyclonal from sheep) are layered onto the gold electrode of 10 MHz piezoelectric crystals, which are precoated with protein A.
6(0,0,1,1) Details
18967431 Turiel E, Fernandez P, Perez-Conde C, Gutierrez AM, Camara C: Flow-through fluorescence immunosensor for atrazine determination. Talanta. 1998 Dec;47(5):1255-61.

A new flow-through fluoroimmunosensor for atrazine determination based on the use of protein A immobilized on controlled pore glass as immunoreactor is reported.
6(0,0,1,1) Details
14558995 Yakovleva J, Davidsson R, Bengtsson M, Laurell T, Emneus J: Microfluidic enzyme immunosensors with immobilised protein A and G using chemiluminescence detection. Biosens Bioelectron. 2003 Oct 30;19(1):21-34.

All immunosensors were capable of detecting atrazine at the sub-microg l (-1) level.
2(0,0,0,2) Details
14568710 Plekhanova YV, Reshetilov AN, Yazynina EV, Zherdev AV, Dzantiev BB: A new assay format for electrochemical immunosensors: polyelectrolyte-based separation on membrane carriers combined with detection of peroxidase activity by pH-sensitive field-effect transistor. Biosens Bioelectron. 2003 Nov 15;19(2):109-14.

The antigens studied were herbicide atrazine and hormone testosterone.
Their specific detection is realised via competitive binding of free and peroxidase-labelled antigens by antibodies integrating with a (staphylococcal protein A-polyanion) conjugate.
2(0,0,0,2) Details
10225515 Choi MJ, Jo Y, Choi J, Kang CY, Han CT: Production and characterization of monoclonal antibodies specific to atrazine group compounds: effects of coating ligand structure on the variation of sensitivity and specificity. J Immunoassay. 1999 Feb-May;20(1-2):57-77.

The protein A affinity purification yielded 0.35-0.65 mg per ml of ascitic fluid from each cell line.
1(0,0,0,1) Details
20334163 Chouhan RS, Rana KV, Suri CR, Thampi RK, Thakur MS: Trace-level detection of atrazine using immuno-chemiluminescence: dipstick and automated flow injection analyses formats. J AOAC Int. 2010 Jan-Feb;93(1):28-35.

In the flow injection analysis (FIA) format, the antibody was immobilized on protein-A sepharose matrix and packed in a glass capillary column, which functioned as an immunoreactor.
1(0,0,0,1) Details
9697344 Narusaka Y, Narusaka M, Kobayashi H, Satoh K: The herbicide-resistant species of the cyanobacterial D1 protein obtained by thorough and random in vitro mutagenesis. Plant Cell Physiol. 1998 Jun;39(6):620-6.

The mutants were screened on the criterion of resistance to either 1 microM DCMU or 10 microM atrazine.
Thus, the result of random mutagenesis basically supports the validity of the proposed structural model for the D1 protein, as well as of the herbicide-binding niche.
1(0,0,0,1) Details
17085033 Zacco E, Galve R, Marco MP, Alegret S, Pividori MI: Electrochemical biosensing of pesticide residues based on affinity biocomposite platforms. Biosens Bioelectron. 2007 Mar 15;22(8):1707-15. Epub 2006 Nov 7.

The better immobilization performance of the anti-atrazine antibodies was achieved by 'dry-assisted affinity' immobilization on Protein A (2%) graphite-epoxy biocomposite (ProtA (2%)-GEB) as a transducer.
82(1,1,1,2) Details