Protein Information

Name ubiquinol cytochrome c reductase (protein family or complex)
Synonyms Ubiquinol cytochrome C reductase complex; ubiquinol cytochrome c reductase; ubiquinol cytochrome c reductases

Compound Information

Name rotenone
CAS

Reference List

PubMed Abstract RScore(About this table)
12515859 Miyadera H, Shiomi K, Ui H, Yamaguchi Y, Masuma R, Tomoda H, Miyoshi H, Osanai A, Kita K, Omura S: Atpenins, potent and specific inhibitors of mitochondrial complex II (succinate-ubiquinone oxidoreductase). Proc Natl Acad Sci U S A. 2003 Jan 21;100(2):473-7. Epub 2003 Jan 6.

The use of specific and potent inhibitors of complex I (NADH-ubiquinone reductase) and complex III (ubiquinol-cytochrome c reductase), such as rotenone and antimycin, respectively, has allowed determination of the role of these enzymes in physiological processes.
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12632423 Maneiro E, Martin MA, de Andres MC, Lopez-Armada MJ, Fernandez-Sueiro JL, del Hoyo P, Galdo F, Arenas J, Blanco FJ: Mitochondrial respiratory activity is altered in osteoarthritic human articular chondrocytes. Arthritis Rheum. 2003 Mar;48(3):700-8.

The activities of mitochondrial respiratory chain complexes (complex I: rotenone-sensitive NADH-coenzyme Q (1) reductase; complex II: succinate dehydrogenase; complex III: antimycin-sensitive ubiquinol cytochrome c reductase; and complex IV: cytochrome c oxidase) and CS were measured in human articular chondrocytes isolated from OA and normal cartilage.
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10370869 Zini R, Morin C, Bertelli A, Bertelli AA, Tillement JP: Effects of resveratrol on the rat brain respiratory chain. Drugs Exp Clin Res. 1999;25(2-3):87-97.

In order to confirm these data, we measured the enzymatic activity of ubiquinol cytochrome c reductase alone and in the presence of resveratrol.
The rate of oxygen consumption by the different complexes was checked using rotenone (2 microM), malonate (10 mM), antimycin A (1 microM), potassium cyanide (KCN) (0.3 mM) and oligomycin (10 microM) to inhibit complexes II, III, IV, V and I, respectively.
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195520 Cadenas E, Boveris A, Ragan CI, Stoppani AO: Production of superoxide radicals and hydrogen peroxide by NADH-ubiquinone reductase and ubiquinol-cytochrome c reductase from beef-heart mitochondria. Arch Biochem Biophys. 1977 Apr 30;180(2):248-57.

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