Protein Information

Name mM 1
Synonyms C myc binding protein; C myc binding protein Mm1; C myc binding protein Mm 1; MM 1; MM1; Myc modulator 1; PFD 5; PFD5…

Compound Information

Name rotenone
CAS

Reference List

PubMed Abstract RScore(About this table)
17900761 Larsen TR, Soderling AS, Caidahl K, Roepstorff P, Gramsbergen JB: Nitration of soluble proteins in organotypic culture models of Parkinson's disease. Neurochem Int. 2008 Feb;52(3):487-94. Epub 2007 Aug 19.

Here we quantified protein-bound 3-nitrotyrosine (3-NT) by a novel gas chromatography/negative chemical ionization tandem mass spectrometry technique and DA and 3,4-dihydroxyphenylalanine (DOPA) by HPLC in tissues or medium of organotypic, mouse mesencephalon cultures after acute or chronic treatments with the peroxynitrite donor 3-morpholino-sydnonimine (SIN-1), the dopaminergic toxin 1-methyl-4-phenylpyridinium (MPP (+)) or the lipophilic complex I inhibitor rotenone.
Incubation with SIN-1 (24 h) or MPP (+) treatments (48 h) caused dose-dependent protein nitration reaching a maximum of eightfold increase by 10 mM SIN-1 or twofold by 10 microM MPP (+), but significant DA depletions occurred at much lower concentrations of MPP (+) (1 microM).
1(0,0,0,1) Details
3988734 Komuniecki R, Fekete S, Thissen-Parra J: Purification and characterization of the 2-methyl branched-chain Acyl-CoA dehydrogenase, an enzyme involved in NADH-dependent enoyl-CoA reduction in anaerobic mitochondria of the nematode, Ascaris suum. J Biol Chem. 1985 Apr 25;260(8):4770-7.

Incubations of A. suum submitochondrial particles, NADH, tiglyl-CoA, purified A. suum electron-transfer flavoprotein, and the 2-methyl branched-chain acyl-CoA dehydrogenase resulted in the rotenone-sensitive, dehydrogenase-dependent formation of 2-methylbutyryl-CoA.
FAD content was estimated to be 0.9 mol/mol of subunit and the absorption coefficient of FAD at 452 nm was 14.1 mM-1 cm-1.
1(0,0,0,1) Details