Protein Information

Name formaldehyde dehydrogenase
Synonyms ADH 3; ADH 5; ADH5; ADH5 protein; ADHX; Alcohol dehydrogenase 5; Alcohol dehydrogenase class 3 chi chain; Alcohol dehydrogenase class III chi chain…

Compound Information

Name rotenone
CAS

Reference List

PubMed Abstract RScore(About this table)
11038276 Lizcano JM, Unzeta M, Tipton KF: A spectrophotometric method for determining the oxidative deamination of methylamine by the amine oxidases. Anal Biochem. 2000 Nov 1;286(1):75-9.

The present work describes a coupled spectrophotometric assay, using formaldehyde dehydrogenase, for the continuous determination of the oxidative deamination of methylamine by semicarbazide-sensitive amine oxidase (SSAO) and its potential use for determining methylamine concentrations in plasma.
Interference from the presence of a rotenone-insensitive NADH oxidase activity in crude tissue homogenates and microsomal fractions can be minimized by pretreating samples with Triton X-100 or substituting NAD (+) by APAD (+) in the coupled assay.
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176156 Cinti DL, Keyes SR, Lemelin MA, Denk H, Schenkman JB: Biochemical properties of rat liver mitochondrial aldehyde dehydrogenase with respect to oxidation of formaldehyde. J Biol Chem. 1976 Mar 25;251(6):1571-7.

The respiratory chain inhibitors, rotenone, and antimycin A plus succinate, strongly inhibited formaldehyde oxidation by apparently the same mechanism, although the crude enzyme preparation (freed from the membrane) was slightly sensitive to rotenone.
Aldehyde (formaldehyde) dehydrogenase activity was completely abolished in the depleted mitochondria, but the enzyme activity was restored to control levels following reincorporation of the pyridine nucleotide.
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