Protein Information

Name estrogen receptor
Synonyms ER; ERA; ER alpha; ERalpha; ESR; ESR 1; ESR1; ESRA…

Compound Information

Name rotenone
CAS

Reference List

PubMed Abstract RScore(About this table)
16410252 Moreira PI, Custodio J, Moreno A, Oliveira CR, Santos MS: Tamoxifen and estradiol interact with the flavin mononucleotide site of complex I leading to mitochondrial failure. J Biol Chem. 2006 Apr 14;281(15):10143-52. Epub 2006 Jan 12.


Furthermore, using two respiratory chain inhibitors, rotenone and diphenyliodonium chloride, we identified the flavin mononucleotide site of complex I as the target of tamoxifen and/or estradiol action (s).
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498075 Olson ME, Sheehan DM: Failure of rotenone to interfere with 17 beta-estradiol action in the rat uterus. Cancer Res. 1979 Nov;39(11):4438-40.

This hypothesis was tested by determining the extent of rotenone inhibition of 17 beta-estradiol binding to the estrogen receptor and of the 17 beta-estradiol-induced uterotrophic response in ovariectomized Sprague-Dawley rats.
113(1,2,2,3) Details
15897899 Felty Q, Singh KP, Roy D: Estrogen-induced G1/S transition of G0-arrested estrogen-dependent breast cancer cells is regulated by mitochondrial oxidant signaling. Oncogene. 2005 Jul 21;24(31):4883-93.


Flow cytometry showed that mitochondrial blockers of protein synthesis (chloramphenicol), transcription and replication (ethidium bromide), and function (rotenone, rhodamine 6G) blocked E2-induced G1 to S transition.
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16608521 Felty Q: Estrogen-induced DNA synthesis in vascular endothelial cells is mediated by ROS signaling. BMC Cardiovasc Disord. 2006 Apr 11;6:16.


E2-induced ROS formation was inhibited to basal levels by cotreatment with the mitochondrial inhibitor rotenone (2 microM) and xanthine oxidase inhibitor allopurinol (50 microM).
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