Protein Information

Name semicarbazide sensitive amine oxidase
Synonyms AOC 3; DAO; Diamine oxidase; Histaminase; Amine oxidase; AOC3; AOC3 Human placenta amine oxidase; Copper amine oxidase…

Compound Information

Name rotenone
CAS

Reference List

PubMed Abstract RScore(About this table)
8397143 Adams JD Jr, Klaidman LK, Leung AC: MPP+ and MPDP+ induced oxygen radical formation with mitochondrial enzymes. Free Radic Biol Med. 1993 Aug;15(2):181-6.

MPP+ may be capable of interacting with submitochondrial particles at a site other than the rotenone site, which results in some formation of oxygen radicals.
Plasma amine oxidase incubations with MPDP+ resulted in O2.- H2O2, and perhaps HO. formation.
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11038276 Lizcano JM, Unzeta M, Tipton KF: A spectrophotometric method for determining the oxidative deamination of methylamine by the amine oxidases. Anal Biochem. 2000 Nov 1;286(1):75-9.

The present work describes a coupled spectrophotometric assay, using formaldehyde dehydrogenase, for the continuous determination of the oxidative deamination of methylamine by semicarbazide-sensitive amine oxidase (SSAO) and its potential use for determining methylamine concentrations in plasma.
Interference from the presence of a rotenone-insensitive NADH oxidase activity in crude tissue homogenates and microsomal fractions can be minimized by pretreating samples with Triton X-100 or substituting NAD (+) by APAD (+) in the coupled assay.
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