| Name | diaphorase |
|---|---|
| Synonyms | Diaphorase; DHQU; DIA 4; DIA4; DT diaphorase; DTD; Diaphorase (NADH/NADPH); Diaphorase (NADH/NADPH) (cytochrome b 5 reductase)… |
| Name | rotenone |
|---|---|
| CAS |
| PubMed | Abstract | RScore(About this table) | |
|---|---|---|---|
| 609151 | Tsuda S, Urakawa N, Fukami J: The inhibitory effect of papaverine on respiration-dependent contracture of guinea pig taenia coli in high-K medium. Jpn J Pharmacol. 1977 Dec;27(6):855-63. The differential effect of papaverine and rotenone on DT diaphorase.. |
13(0,0,2,3) | Details |
| 1777518 | Kolesova GM, Karnaukhova LV, Iaguzhinskii LS: [Interaction of and duroquinone with Q-cycle during DT-diaphorase function]. Biokhimiia. 1991 Oct;56(10):1779-86. |
7(0,0,0,7) | Details |
| 6094566 | Moreno SN, Mason RP, Docampo R: Ca2+ and Mg2+-enhanced reduction of arsenazo III to its anion free radical metabolite and generation of by an outer mitochondrial membrane azoreductase. J Biol Chem. 1984 Dec 10;259(23):14609-16. |
3(0,0,0,3) | Details |
| 2421769 | Malviya AN, Mandel P, Mersel M: The nature of DT-diaphorase (EC 1.6.99.2) activity in plasma membrane of astrocytes in primary cultures. Biochim Biophys Acta. 1986 Apr 24;849(2):288-92. |
3(0,0,0,3) | Details |
| 19096101 | Takahashi T, Okuno M, Okamoto T, Kishi T: -dependent reductase is the main enzyme responsible for the reduction of non-mitochondrial in cells. Biofactors. 2008;32(1-4):59-70. We purified an -dependent reductase - reductase) in rat liver cytosol and compared its enzymatic properties with those of the other CoQ10 reductases such as acceptor oxidoreductase 1 (NQO1), dehydrogenase, thioredoxine reductase and glutathione reductase. |
2(0,0,0,2) | Details |
| 8882715 | Takahashi T, Okamoto T, Kishi T: Characterization of -dependent ubiquinone reductase activity in rat liver cytosol: effect of various factors on -reducing activity and discrimination from other quinone reductases. J Biochem. 1996 Feb;119(2):256-63. Rotenone, antimycin A, and KCN, which inhibit mitochondrial and microsomal electron transfer enzymes, superoxide dismutase, and acetylated cytochrome c had no effect on the -UQ reductase activity. These results indicated that the -UQ reductase in rat liver cytosol is a flavoprotein that reduces UQ-10 by a two-electron reduction mechanism and is distinguishable from known microsomal and mitochondrial enzymes, as well as DT-diaphorase [EC 1.6.99.2]. |
1(0,0,0,1) | Details |
| 20227863 | He M, Siow RC, Sugden D, Gao L, Cheng X, Mann GE: Induction of HO-1 and redox signaling in endothelial cells by advanced glycation end products: A role for Nrf2 in vascular protection in diabetes. Nutr Metab Cardiovasc Dis. 2010 Mar 12. We have investigated the mechanisms by which AGE-modified bovine albumin (AGE-BSA) induces reactive species (ROS) generation, leading to nuclear factor-erythroid 2-related factor (Nrf2) dependent induction of the antioxidant genes heme oxygenase-1 (HO-1) and NADPH:quinone oxidoreductase 1 (NQO1) in bovine aortic endothelial cells. Inhibition of ROS production with the scavenger Tiron or inhibitors of flavoproteins (diphenylene iodonium) and oxidase (apocynin), but not eNOS (l-NAME) or mitochondria complex I (rotenone) abrogated HO-1 induction by AGE-BSA. |
1(0,0,0,1) | Details |
| 17360324 | Liu Y, Kern JT, Walker JR, Johnson JA, Schultz PG, Luesch H: A genomic screen for activators of the antioxidant response element. Proc Natl Acad Sci U S A. 2007 Mar 20;104(12):5205-10. Epub 2007 Mar 12. Overexpression of SQSTM1 and DPP3 in IMR-32 cells stimulated NF-E2-related factor 2 (NRF2) nuclear translocation and led to increased levels of NAD (P) H:quinone oxidoreductase 1, a protein which is transcriptionally regulated by the ARE. Overexpression of these cDNAs conferred partial resistance to peroxide or rotenone-induced toxicity, consistent with the induction of antioxidant and phase II detoxification enzymes, which can protect from oxidative stress. |
2(0,0,0,2) | Details |
| 1002973 | Benkoel L, Chamlian A, Barrat E, Laffargue P: The use of ferricyanide for the electron microscopic demonstration of dehydrogenases in human steroidogenic cells. J Histochem Cytochem. 1976 Nov;24(11):1194-203. The precise localization of various reactions in different electron transfer chains was determined by using different ferricyanide concentrations and intermediate electron-carriers such as or exogenous and reduced form-diaphorase. The use of respiratory chain inhibitors such as rotenone or antimycine A confirmed these data. |
1(0,0,0,1) | Details |
| 15170383 | Elanskaya IV, Timofeev KN, Grivennikova VG, Kuznetsova GV, Davletshina LN, Lukashev EP, Yaminsky FV: Reduction of photosystem I reaction center in DrgA mutant of the cyanobacterium Synechocystis sp. Biochemistry. 2004 Apr;69(4):445-54. PCC 6803 lacking soluble NAD (P) H:quinone oxidoreductase.. PCC 6803 cells to oxidize under heterotrophic conditions and did not impair the NDH-1-dependent, rotenone-inhibited electron transfer from to P700+ in thylakoid membranes of the cyanobacterium. |
1(0,0,0,1) | Details |
| 12950443 | He Y, Imam SZ, Dong Z, Jankovic J, Ali SF, Appel SH, Le W: Role of in rotenone-induced nigro-striatal injury. J Neurochem. 2003 Sep;86(6):1338-45. Furthermore, a significant (37%) increase in the number of cells positive for diaphorase -d) in the striatum was observed, accompanied by a 83% increase in synthase (NOS) activity and a significant increase in the production of (3-NT). |
1(0,0,0,1) | Details |
| 15893762 | Shneyvays V, Leshem D, Shmist Y, Zinman T, Shainberg A: Effects of and its derivative on cultured cardiomyocytes with mitochondrial disorders. J Mol Cell Cardiol. 2005 Jul;39(1):149-58. and more effectively, AK-135, restored the electron flow in defective respiratory chain (hypoxia or rotenone) systems. In cultures pretreated with 4 microM dicumarol (DT-diaphorase inhibitor), the protective effect of AK-135 and was abolished completely (1.67+/-1.43 and 2.97+/-0.57 nmol/mg protein, respectively). |
1(0,0,0,1) | Details |
| 5732503 | Jurtshuk P, Harper L: Oxidation of D (minus) lactate by the electron transport fraction of Azotobacter vinelandii. J Bacteriol. 1968 Sep;96(3):678-86. The d (-) lactic phenazine methosulfate and menadione reductases were sensitive only to dicumarol and TFTB. The d (-) lactic oxidase activity was sensitive to electron transport inhibitors, i.e., chlorpromazine, antimycin A, 2-n-heptyl-4-hydroxyquinoline-N-oxide, rotenone, dicumarol, and and to a small extent to 4,4,4-trifluoro-1-(2-thienyl)-1,3-butane-dione (TFTB) and Amytal. |
1(0,0,0,1) | Details |
| 823748 | Schewe T, Hiebsch C, Halangk W: [Action of the systemic fungicide dexon on several NADH dehydrogenases] . Acta Biol Med Ger. 1975;34(11-12):1767-75. Soluble -cytochrome c-oxidoreductase (MAHLER) and rotenone-insensitive ubiquinone reductase are also inhibited by dexon. In addition, dexon inhibits selectively the NADH dehydrogenase of the DT diaphorase (ERNSTER) from rat liver cytosol. |
1(0,0,0,1) | Details |
| 11167513 | Piasecka M, Wenda-Rozewicka L, Ogonski T: Computerized analysis of cytochemical reactions for dehydrogenases and oxygraphic studies as methods to evaluate the function of the mitochondrial sheath in rat spermatozoa. Andrologia. 2001 Jan;33(1):1-12. Cytochemical reactions for mitochondrial -dependent dehydrogenases (diaphorase/ which is related to flavoprotein), NAD-dependent dehydrogenases and succinate dehydrogenase were carried out in rat spermatozoa. |
1(0,0,0,1) | Details |
| 18140 | Ragan CI, Bloxham DP: Specific labelling of a constituent polypeptide of bovine heart mitochondrial -ubiquinone reductase by the inhibitor diphenyleneiodonium. Biochem J. 1977 Jun 1;163(3):605-15. Rotenone increased the apparent affinity of diphenyleneiodonium for the 23500-mol.wt. polypeptide without affecting the maximum incorporation. 8. -ubiquinone-1 and -menadione reductase activities of Complex I were inhibited by diphenyleneiodonium (apparent Ki 23 and 30 nmol/mg of protein respectively). |
1(0,0,0,1) | Details |
| 4291554 | Hall CL, Crane FL: Variation in inhibitor sensitivity of -menadione reductase from mitochondria. Biochem Biophys Res Commun. 1967 Jan 23;26(2):138-47. |
1(0,0,0,1) | Details |
| 7639706 | Takahashi T, Yamaguchi T, Shitashige M, Okamoto T, Kishi T: Reduction of in membrane lipids by rat liver cytosol and its involvement in the cellular defence system against lipid peroxidation. Biochem J. 1995 Aug 1;309 ( Pt 3):883-90. This -dependent UQ reductase -UQ reductase) activity that was not inhibited by antimycin A and rotenone, was located mainly in the cytosol fraction and its activity accounted for 68% of that of the homogenates. The -UQ reductase activity was clearly separated from DT-diaphorase (EC 1.6.99.2) activity by means of Cibacron Blue-immobilized Bio-Gel A-5m chromatography. |
1(0,0,0,1) | Details |
| 17967736 | Paris I, Cardenas S, Lozano J, Perez-Pastene C, Graumann R, Riveros A, Caviedes P, Segura-Aguilar J: Aminochrome as a preclinical experimental model to study degeneration of dopaminergic neurons in Parkinson's disease. Neurotox Res. 2007 Sep;12(2):125-34. Different model neurotoxins have been used as preclinical experimental models to study the neurodegenerative process in PD, such as (6-OHDA), 1-methyl-4-phenyl-1,2,3,6-tetrahydropyridine (MPTP), and rotenone. These two pathways of aminochrome are prevented by DT-diaphorase, an enzyme that effectively reduces aminochrome with two-electrons preventing both aminochrome one-electron reduction or formation alpha synuclein protofibrils. |
1(0,0,0,1) | Details |
| 17601793 | Merker MP, Audi SH, Lindemer BJ, Krenz GS, Bongard RD: Role of mitochondrial electron transport complex I in reduction by intact pulmonary arterial endothelial cells and the effect of hyperoxia. Am J Physiol Lung Cell Mol Physiol. 2007 Sep;293(3):L809-19. Epub 2007 Jun 29. The mitochondrial electron transport complex I inhibitor rotenone decreased the CoQ (1) reduction rate by 85% in the normoxic cells and 44% in the hyperoxia-exposed cells. |
0(0,0,0,0) | Details |
| 9625705 | Bernhard Teicher H, Vibe Scheller H: The NAD (P) H dehydrogenase in barley thylakoids is photoactivatable and uses as well as . Plant Physiol. 1998 Jun;117(2):525-32. The enzyme was sensitive to rotenone, confirming the involvement of a complex I-type enzyme. |
0(0,0,0,0) | Details |
| 3020812 | Moore GA, O'Brien PJ, Orrenius S: -induced Ca2+ release from rat-liver mitochondria is caused by NAD (P) H oxidation. Xenobiotica. 1986 Sep;16(9):873-82. Incubation of rat-liver mitochondria with in the presence of and rotenone resulted in rapid and NAD (P) H oxidation followed by Ca2+ release and mitochondrial swelling. |
0(0,0,0,0) | Details |
| 14766796 | Lapointe N, St-Hilaire M, Martinoli MG, Blanchet J, Gould P, Rouillard C, Cicchetti F: Rotenone induces non-specific central nervous system and systemic toxicity. FASEB J. 2004 Apr;18(6):717-9. Epub 2004 Feb 6. |
0(0,0,0,0) | Details |
| 9211432 | Aitken RJ, Fisher HM, Fulton N, Gomez E, Knox W, Lewis B, Irvine S: Reactive species generation by human spermatozoa is induced by exogenous and inhibited by the flavoprotein inhibitors diphenylene iodonium and quinacrine. Mol Reprod Dev. 1997 Aug;47(4):468-82. Addition of to viable populations of motile spermatozoa induced a sudden dose-dependent increase in the rate of generation via mechanisms that could not be disrupted by inhibitors of the mitochondrial electron transport chain (antimycin A, rotenone, carbonyl m-chlorophenylhydrazone [CCCP], and azide), diaphorase (dicoumarol) xanthine oxidase (allopurinol), or lactic acid dehydrogenase oxamate). |
0(0,0,0,0) | Details |
| 18703762 | Audi SH, Merker MP, Krenz GS, Ahuja T, Roerig DL, Bongard RD: redox metabolism during passage through the rat pulmonary circulation and the effect of hyperoxia. J Appl Physiol. 2008 Oct;105(4):1114-26. Epub 2008 Aug 14. In normoxic lungs, CoQ (1) H (2) efflux rates when CoQ (1) was infused decreased by 58 and 33% in the presence of rotenone (mitochondrial complex I inhibitor) and dicumarol [NAD (P) H-quinone oxidoreductase 1 (NQO1) inhibitor], respectively. |
32(0,1,1,2) | Details |
| 12069106 | Chan TS, Teng S, Wilson JX, Galati G, Khan S, O'Brien PJ: cytoprotective mechanisms for mitochondrial complex I cytopathies involves NAD (P) H: quinone oxidoreductase 1 (NQO1). Free Radic Res. 2002 Apr;36(4):421-7. This suggests that the CoQ1H2 formed by NQO1 reduced complex III and acted as an electron bypass of the rotenone block. |
84(1,1,1,4) | Details |
| 8268305 | Kolesova GM, Karnaukhova LV, Segal' NK, Iaguzhinskii LS: [The effect of inhibitors of the Q-cycle on cyano-resistant oxidation of by rat liver mitochondria in the presence of Biokhimiia. 1993 Oct;58(10):1630-40. In the presence of rotenone, when is reduced by DT-diaphorase, the rate of cyano-resistant respiration decreases approximately twofold; its sensitivity towards myxothiazole and antimycin drops down to 40%. |
81(1,1,1,1) | Details |
| 10841308 | de Halac IN, Bacman SR, de Kremer RD: Histoenzymology of oxidases and dehydrogenases in peripheral blood lymphocytes and monocytes for the study of mitochondrial oxidative phosphorylation. Histochem J. 2000 Mar;32(3):133-7. The specificity of each histoenzymological reaction was tested using a specific respiratory chain inhibitor: rotenone for diaphorase, thenoyltrifluoroacetone for succinate dehydrogenase, for cytochrome c oxidase and oligomycin for ATPase. |
31(0,1,1,1) | Details |