Protein Information

Name N acetyl beta glucosaminidase
Synonyms Beta N acetylhexosaminidase; Beta hexosaminidase beta chain; Beta hexosaminidase beta chain precursor; Cervical cancer proto oncogene 7; ENC 1AS; HCC 7; HCC7; HEXB…

Compound Information

Name rotenone
CAS

Reference List

PubMed Abstract RScore(About this table)
223146 Hayes LW, Goguen CA, Stevens AL, Magargal WW, Slakey LL: Enzyme activities in endothelial cells and smooth muscle cells from swine aorta. Proc Natl Acad Sci U S A. 1979 Jun;76(6):2532-5.

In populations of cultured arterial endothelial and smooth muscle cells grown under the same conditions, we have measured the total activity per cell of 10 enzymes commonly used as "markers" for subcellular organelles: NADH: ferricyanide reductase, NADH:cytochrome c reductase (rotenone insensitive).
NADPH:cytochrome c reductase, alpha-glucosidase, 5'-nucleotidase, alkaline phosphodiesterase I, cytochrome oxidase, monoamine oxidase, cathepsin D, and N-acetyl-beta-glucosaminidase.
1(0,0,0,1) Details
213202 Kennett FF, Weglicki WB: Effects of well-defined ischemia on myocardial lysosomal and microsomal enzymes in a canine model. Circ Res. 1978 Nov;43(5):750-8.

Significant decreases (P less than 0.001) in content of protein were seen in all post 1,000 g pellets and supernatant fluids in the L-ischemia zones; particulate lysosomal enzymatic activity was significantly decreased (P less than 0.001) in all four post 1,000 g pellets (2,500 g to 140,000 g) of the L-ischemic areas (for N-acetyl-beta-glucosaminidase and beta-glucuronidase).
In addition, about 45% of the total activity of the microsomal marker enzyme, rotenone-insensitive NADH cytochrome C reductase (RINCR), was found in the 140,000 g pellet of H-control tissue (9.9 micronmol/min per g); this activity fell to 8.1 micronmol/min per g in M-ischemic areas (P less than 0.001) and to 5.3 micronmol/min per g in L-ischemic areas (P less than 0.001).
1(0,0,0,1) Details
7423051 Weglicki WB, Kennett FF, Ruth RC: Lysosomal changes in an animal model of myocardial ischemia. Adv Myocardiol. 1980;2:371-81.

It also permitted great enrichment of a membrane-bound enzyme localized to a microsomal fraction, rotenone-insensitive NADH-cytochrome c reductase (RINCR).
N-Acetyl-beta-glucosaminidase (NAG) was the most reliable lysosomal marker enzyme; this was depleted significantly (P < 0.001) in particulate lysosomal fractions; significant increases in the supernatant (P < 0.001) were found in areas of ischemia that were less than 25% of the control MBF.
1(0,0,0,1) Details