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Hagedorn CH: Demonstration of a NADPH-linked delta 1-pyrroline-5-carboxylate-proline shuttle in a cell-free rat liver system. Biochim Biophys Acta. 1986 Oct 29;884(1):11-7.
A phosphate-free buffer with high concentrations of triethanolamine and 2-mercaptoethanol prevented the cold inactivation of pyrroline-5-carboxylate reductase (EC 1.5.1.2) in liver extracts.
Rotenone was required for maximal activity, suggesting that inhibition of delta 1-pyrroline-5-carboxylate efflux would be required for significant shuttle activity in vivo. |
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