Protein Information

Name protein kinase A (protein family or complex)
Synonyms Protein kinase A; cAMP dependent protein kinase; cAMP dependent protein kinases

Compound Information

Name IBA
CAS

Reference List

PubMed Abstract RScore(About this table)
1313168 Doupnik CA, Pun RY: Cyclic AMP-dependent phosphorylation modifies the gating properties of L-type Ca2+ channels in bovine adrenal chromaffin cells. Pflugers Arch. 1992 Jan;420(1):61-71.

Extracellular perfusion with the membrane-permeant activator of cAMP-dependent protein kinase, 8-bromo (8-Br)-cAMP (1 mM), caused a 49%, 29%, and 21% increase in Ca2+ current (ICa) amplitudes evoked by voltage steps to 0, +10, and +20 mV respectively (mean values from eight cells, p less than or equal to 0.05).
The membrane potential for half activation (V1/2) was also significantly shifted 6 mV more negative for IBa (mean, n = 8).
1(0,0,0,1) Details
9594024 Allen TJ, Mikala G: Effects of temperature on human L-type cardiac Ca2+ channels expressed in Xenopus oocytes. Pflugers Arch. 1998 Jul;436(2):238-47.

Temperature normally affects peak L-type Ca2+ channel (CaCh) current with a temperature coefficient (Q10) of between 1.8 and 3.5; in cardiomyocytes attenuating protein kinase A activity increases Q10 whilst activating it lowers Q10.
Peak inward currents (IBa) through expressed CaChs (i.e. alpha1C alpha2/deltaa beta1b) exhibited a Q10 of 5.8+/-0.4 when examined between 15 and 25 degreesC.
1(0,0,0,1) Details
1371671 Lory P, Nargeot J: Cyclic AMP-dependent modulation of cardiac Ca channels expressed in Xenopus laevis oocytes. Biochem Biophys Res Commun. 1992 Feb 14;182(3):1059-65.


Inhibition of protein kinase A (PKA) dramatically reduced the Ba current amplitude, indicating that cAMP-dependent modulation plays an important role in maintaining the basal activity of expressed Ca channels.
1(0,0,0,1) Details
7491257 Zong X, Schreieck J, Mehrke G, Welling A, Schuster A, Bosse E, Flockerzi V, Hofmann F: On the regulation of the expressed L-type calcium channel by cAMP-dependent phosphorylation. Pflugers Arch. 1995 Jul;430(3):340-7.


The peak Ba2+ current (IBa) of these cells was not affected significantly by internal dialysis with 0.1 mM cAMP-dependent protein kinase inhibitor peptide (mPKI), 25 microM cAMP-dependent protein kinase catalytic subunit (PKA), or a combination of 25 microM PKA and 1 microM okadaic acid.
0(0,0,0,0) Details
9789110 Sunagawa M, Yokoshiki H, Seki T, Sperelakis N: Intracellular application of calmidazolium increases Ca2+ current through activation of protein kinase A in cultured vascular smooth muscle cells. J Vasc Res. 1998 Sep-Oct;35(5):303-9.

On the other hand, the CMZ stimulation of IBa was prevented by both H-7, a nonspecific protein kinase inhibitor, and H-89, a specific inhibitor of protein kinase A (PK-A).
82(1,1,1,2) Details
7900772 Yuan W, Bers DM: Protein kinase inhibitor H-89 reverses forskolin stimulation of cardiac L-type calcium current. Am J Physiol. 1995 Mar;268(3 Pt 1):C651-9.

The acceleration is likely to be due to greater Ca-dependent inactivation of ICa, where the slowing of IBa inactivation may be due to protein kinase A-dependent slowing of Em-dependent inactivation.
81(1,1,1,1) Details
10226153 Zhong J, Dessauer CW, Keef KD, Hume JR: Regulation of L-type Ca2+ channels in rabbit portal vein by G protein alphas and betagamma subunits. J Physiol. 1999 May 15;517 ( Pt 1):109-20.

The stimulatory effect of Galphas on peak IBa was entirely abolished by the protein kinase A inhibitor Rp-8-Br-cAMPS, or the adenylyl cyclase inhibitor SQ 22536.
32(0,1,1,2) Details
9062905 Van Coppenolle F, Ahidouch A, Guilbault P, Ouadid H: Regulation of endogenous Ca2+ channels by cyclic AMP and cyclic GMP-dependent protein kinases in Pleurodeles oocytes. Mol Cell Biochem. 1997 Mar;168(1-2):155-61.

This effect on IBa was blocked by the injection of protein kinase A inhibitor.
32(0,1,1,2) Details
17397827 Walsh KB, Zhang J, Fuseler JW, Hilliard N, Hockerman GH: Adenoviral-mediated expression of dihydropyridine-insensitive L-type calcium channels in cardiac ventricular myocytes and fibroblasts. Eur J Pharmacol. 2007 Jun 22;565(1-3):7-16. Epub 2007 Mar 3.

Furthermore, addition of 2 microM forskolin and 100 microM 3-isobutyl-1-methylxanthine (IBMX), to stimulate protein kinase A, strongly increased IBa in the AdCaV1.2DHPi-infected cells.
6(0,0,1,1) Details
9490807 Allen TJ, Mikala G, Wu X, Dolphin AC: Effects of 2,3-butanedione monoxime (BDM) on calcium channels expressed in Xenopus oocytes. J Physiol. 1998 Apr 1;508 ( Pt 1):1-14.

In previous studies on L-type Ca2+ channel currents in cardiomyocytes and dorsal root ganglia, the inhibitory effects of BDM were attenuated by activation of protein kinase A. 2.
Ba2+ currents (IBa) through a human wild-type L-type Ca2+ channel complex (i.e. halpha1C, alpha2-deltaa and hbeta1b) are inhibited by BDM with an IC50 of 16 mM, with 10 mM producing a 36.1 +/- 2.2 % inhibition.
1(0,0,0,1) Details
7535119 Kaneko S, Fukuda K, Yada N, Akaike A, Mori Y, Satoh M: Ca2+ channel inhibition by kappa opioid receptors expressed in Xenopus oocytes. Neuroreport. 1994 Dec 20;5(18):2506-8.

Perfusion of the oocytes with U50488H inhibited depolarization-evoked Ba2+ current (IBa) in a reversible manner, showing maximal inhibition of 25% at 1 microM (IC50 = 31 nM).
These findings suggest that kappa opioid receptors inhibit the activity of neuronal Ca2+ channels via GTP-binding proteins, without the participation of protein kinase A or C.
1(0,0,0,1) Details
11179395 Zhong J, Hume JR, Keef KD: beta-Adrenergic receptor stimulation of L-type Ca2+ channels in rabbit portal vein myocytes involves both alphas and betagamma G protein subunits. J Physiol. 2001 Feb 15;531(Pt 1):105-15.

Previous studies have shown that purified G protein alphas and betagamma subunits stimulate vascular L-type Ca2+ channels through protein kinase A and C (PKA and PKC), respectively.
Peak Ba2+ current (IBa) in freshly isolated rabbit portal vein smooth muscle cells was significantly increased by bath application of 0.5 microM isoproterenol (isoprenaline; ISO) when measured using the whole-cell patch clamp method (53 +/- 3 % increase, n = 15).
1(0,0,0,1) Details
2435866 Leonard JP, Nargeot J, Snutch TP, Davidson N, Lester HA: Ca channels induced in Xenopus oocytes by rat brain mRNA. J Neurosci. 1987 Mar;7(3):875-81.


IBa in noninjected oocytes was often undetectable, but ranged up to 50 nA (22 +/- 4 nA, n = 21).
0(0,0,0,0) Details