| 17121357 |
Zhu J, Zhang B, Yan X, Lao X, Yu H: [Encapsulating hepatocytes with chitosan in physiological conditions] . Sheng Wu Yi Xue Gong Cheng Xue Za Zhi. 2006 Oct;23(5):1070-4.
Prepared from 15.3% N-acetylated chitosan (FNC), half N-acetylated chitosan (HNC) possesses a good solubility in a weak basic solution, guaranteeing the formation of microcapsules by the coacervating reaction between HNC and methacrylic acid (MAA)-hydroxyethyl methacrylate (HEMA)-methyl methacrylate (MMA) (MAA-HEMA-MMA) terpolymer under physiological conditions. When hepatocytes were encapsulated in such 3-dimensional microenvironment, as compared to monolayer culture, cell functions, including P450 activity, urea production and albumin release, were well supported. The prepared microcapsules have good mechanical stability and permeability. |
31(0,1,1,1) |