| 18763191 |
Jiang D, Jiang Z, Li Z, Zhang Y: Suppression of the production of extracellular matrix and alpha-smooth muscle actin induced by transforming growth factor-beta1 in fibroblasts of the flexor tendon sheath by hepatocyte growth factor. J Cell Biol. 2010 Feb 8;188(3):383-99. Epub 2010 Feb 1.
We examined the effectiveness of hepatocyte growth factor (HGF) in blocking production of transforming growth factor (TGF)-beta1-induced collagen I, fibronectin, and alpha-smooth muscle actin (alpha-SMA) in the flexor tendon sheath of rabbits in vitro. Fibroblasts were obtained from the sheaths. Cell culture was supplemented with TGF-beta1 5 ng/ml and increasing doses of HGF (10-40 ng/ml). The production of collagen I and fibronectin in supernatants culture were examined using an enzyme-linked immunosorbent assay (ELISA). alpha-SMA expression was assessed by western blot. TGF-beta1 stimulated production of collagen I, fibronectin, and alpha-SMA greatly, while HGF significantly (p <0.05) reduced production of all components induced by TGF-beta1 in a dose-dependent manner. This suggests that HGF effectively antagonises the action of TGF-beta1 in cultured fibroblasts from flexor tendon sheaths. The results provide a cellular and molecular basis for HGF acting as a therapeutic agent for adhesions in flexor tendons. |
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