| 19167828 |
Zhang Z, Garron TM, Li XJ, Liu Y, Zhang X, Li YY, Xu WS: Recombinant human decorin inhibits TGF-beta1-induced contraction of collagen lattice by hypertrophic scar fibroblasts. Am J Respir Cell Mol Biol. 2009 Jun;40(6):643-53. Epub 2008 Nov 6.
Decorin was reported to bind transforming growth factor-beta (TGF-beta (1)) and neutralise some of its activity as a key regulator of wound contraction and hypertrophic scar formation. In this study, we investigated whether recombinant human decorin affected TGF-beta (1)-induced fibroblast contractile activity, by using fibroblast-populated collagen lattice with decorin added to the collagen gel. Hypertrophic scar fibroblasts showed greater basal contraction of collagen gels than normal fibroblasts, and the addition of TGF-beta (1) significantly enhanced this. Decorin inhibited both the basal and TGF-beta (1)-enhanced contraction of collagen gel by both normal and hypertrophic scar fibroblasts. Decorin also inhibited TGF-beta (1)-induced alpha-smooth muscle actin (alpha-SMA), plasminogen activator inhibitor-1 (PAI-1) protein and mRNA expressions in normal and hypertrophic scar fibroblasts. These results suggest that decorin may have therapeutic potential for excessive skin contraction as observed in hypertrophic scarring. |
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