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Gogol EV, Evtugyn GA, Marty JL, Budnikov HC, Winter VG: Amperometric biosensors based on nafion coated screen-printed electrodes for the determination of cholinesterase inhibitors. Talanta. 2000 Nov 1;53(2):379-89.
Screen-printed electrodes coated with the nafion layer have been investigated for cholinesterase biosensor design. The butyrylcholinesterase (ChE) from horse serum was immobilised onto the nafion layer by cross-linking with glutaraldehyde vapours. The biosensors obtained showed better long-term stability and lower working potential in comparison to those obtained with no nafion coating. The sensitivity of a biosensor toward organophosphate pesticides is not affected by the nafion coating. The detection limits were found to be 3.5x10 (-7) M for trichlorfon and 1.5x10 (-7) M for coumaphos. |
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