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Gardner G: Azidoatrazine: Photoaffinity Label for the Site of Triazine Herbicide Action in Chloroplasts. 2363: id: 5866589 Error occurred: Error 11 (Resource temporarily unavailable)Science. 1981 Feb 27;211(4485):937-940.
Binding of the 4-azido analog of the herbicide atrazine to pea chloroplast membranes was compared with that of atrazine. When [(14) C] azidoatrazine was treated with 300-nanometer ultraviolet light in situ, reversibility of binding was lost in proportion to the duration of irradiation. Sodium dodecyl sulfate-polyacrylamide gel electrophoresis of chloroplast membranes irradiated in the presence of [(l4) C]-azidoatrazine indicated radioactivity in only one region, corresponding to a protein with a molecular weight of approximately 32,000. Azidoatrazine is a photoaffinity reagent for the triazine binding site in chloroplasts and serves as a label to identify this site, which may be the apoprotein of the secondary electron acceptor in photosystem II. |
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