| 15357675 |
Lew TS, Chang CS, Fang KP, Chen CY, Chen CH, Lin SC: The involvement of K (v) 3.4 voltage-gated potassium channel in the growth of an oral squamous cell carcinoma cell line. J Oral Pathol Med. 2004 Oct;33(9):543-9.
BACKGROUND: In our previous study, an A-type voltage-gated K (+) channel, K (v) 3.4, was found more frequently expressed in oral squamous cell carcinoma (OSCC) when compared with non-cancerous matched oral tissue. An OSCC cell line, OECM-1, was found to have moderate level of K (v) 3.4 expression. METHODS: To further elucidate the roles of K (v) 3.4 for the involvement of neoplastic process, we amplified K (v) 3.4 coding sequence by reverse transcriptase polymerase chain reaction (RT-PCR), constructed an expression vector carrying this sequence and then stably transfected into OECM-1 OSCC cells. RESULTS: We demonstrated the integration and constitutive expression of K (v) 3.4 in the cell. A unique A-type current elicited by such expression in OECM-1 cells was defined by patch clamp analysis. This current pattern can be reversibly blocked by an A-type K (+) channel blocker 2 mM 4-aminopyridine (4-AP). The acquisition of K (v) 3.4 activity in OECM-1 cells bestowed growth advantage. However, in 3T3 cell, transfected K (v) 3.4 caused only limited increase of growth without forming transformation foci. CONCLUSION: The present study established a stable keratinocyte system carrying functional K (v) 3.4 and increase of growth, by which the anti-K (v) 3.4 modalities for potential OSCC control can be further investigated. |
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