Protein Information

ID 103
Name adenylyl cyclase
Synonyms ADCY 7; ATP pyrophosphate lyase; Adenylyl cyclase; ADCY7; ADCY7 protein; ATP pyrophosphate lyase 7; Adenylate cyclase 7; Adenylate cyclase type 7…

Compound Information

ID 1388
Name sodium fluoride
CAS sodium fluoride (NaF)

Reference

PubMed Abstract RScore(About this table)
11032978 Emamghoreishi M, Li PP, Schlichter L, Parikh SV, Cooke R, Warsh JJ: Associated disturbances in calcium homeostasis and G protein-mediated cAMP signaling in bipolar I disorder. Biol Psychiatry. 2000 Oct 1;48(7):665-73.
BACKGROUND: Evidence of extensive cross-talk between calcium (Ca (2+))- and cAMP-mediated signaling systems suggests that previously reported abnormalities in Ca (2+) homeostasis in bipolar I (BP-I) patients may be linked to disturbances in the function of G proteins that mediate cAMP signaling. METHODS: To test this hypothesis, the beta-adrenergic agonist, isoproterenol, and the G protein activator, sodium fluoride (NaF), were used to stimulate cAMP production in B lymphoblasts from healthy and BP-I subjects phenotyped on basal intracellular calcium concentration ([Ca (2+)](B)). cAMP was measured by radioimmunoassay and [Ca (2+)](B) by ratiometric fluorometry with fura-2. RESULTS: Isoproterenol- (10 microM) stimulated cAMP formation was lower in intact B lymphoblasts from BP-I patients with high [Ca (2+)](B) (>/= 2 SD above the mean concentration of healthy subjects) compared with patients having normal B lymphoblast [Ca (2+)](B) and with healthy subjects. Although basal and NaF-stimulated cAMP production was greater in B lymphoblast membranes from male BP-I patients with high versus normal [Ca (2+)](B), there were no differences in the percent stimulation. This suggests the differences in NaF response resulted from higher basal adenylyl cyclase activity. CONCLUSIONS: These findings suggest that trait-dependent disturbances in processes regulating beta-adrenergic receptor sensitivity and G protein-mediated cAMP signaling occur in conjunction with altered Ca (2+) homeostasis in those BP-I patients with high B lymphoblast [Ca (2+)](B).
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