| 17327035 |
Weng ZP, Wang B, Wang SL, Cui N, Pan XY: [Influence of 4-aminopyridine on cell proliferation through inhibiting voltage-gated K+ channel in ovarian cancer]. Zhonghua Fu Chan Ke Za Zhi. 2006 Nov;41(11):762-5.
OBJECTIVE: To study the inhibition of cell proliferation by 4-aminopyridine (4-AP) in the human ovarian cancer cell SKOV3. METHODS: The expression of voltage-gated K (+) channel on the SKOV3 cell line was detected by reverse transcription polymerase chain reaction. Inhibition of voltage-gated K (+) current by 4-AP through the whole-cell patch-clamp technique on SKOV3 cell line was recorded. The influence on the cell-cycle of the SKOV3 cell line by 4-AP was observed by flow cytometry and the inhibition of the cell proliferation was studied using methyl thiazolyl tetrazolium (MTT) method. RESULTS: Voltage-gated K (+) channel was expressed in SKOV3 cell. Exposure of the SKOV3 cell to 5 mmol/L 4-AP reduced K (+) current by 52.5%. The 4-AP at 5 mmol/L significantly effected the progression of cell cycle with a 72 h treatment of SKOV3 cell. Exponentially growing cells without inhibitors had a distribution of 39.7% in G (0)/G (1) phase, 57.3% in S phase, and 3.0% in G (2)/M phase. In 4-AP-treated cells, the proportion of G (0)/G (1) cells increased significantly to 62.3% (P < 0.05), while there was a significant decrease in S phase cells (36.2%, P < 0.05) and G (2)/M phase (1.4%, P < 0.05). Incubation of the SKOV3 cells with 0.1, 1, 5, 10, 15, 20 mmol/L 4-AP resulted in a concentration-dependent reduction in the number of viable cells as compared with the control, and the inhibition rate was 17.5%, 35.0%, 54.6%, 69.1%, 71.2%, 72.8% respectively (vs control, P < 0.01). CONCLUSION: The voltage-gated K (+) channels expressed by SKOV3 play an important role in SKOV3 cell proliferation. |
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