| 12958834 |
Liu XS, Xu YJ, Zhang ZX, Ni W: [Effects of potassium channel blockers on the proliferation of rat bronchial smooth muscle cells]. Yao Xue Xue Bao. 2003 May;38(5):333-6.
AIM: To investigate the effects of blockers of the three kinds of potassium channels: voltage-dependent K+ channel (KV), calcium-activated K+ channel (KCa) and ATP-sensitive K+ channel (KATP), on the proliferation of rat bronchial smooth muscle cells (BSMCs). METHODS: The effects of three kinds of potassium channel blockers on the proliferation of BSMCs were detected by MTT method, immunocytochemistry staining and flow-cytometry. Their effects on the dynamic changes of Ca2+ concentration in BSMCs were investigated by the fluorophotometry of Fura-2/AM. RESULTS: The KV blocker 4-aminopyridine (4-AP) was shown to significantly increase the expression of proliferating cell nucleus antigen in cultured rat BSMCs (P < 0.01), but the KCa blocker tetraethylammonium (TEA) and KATP blocker glibenclamide (Glib) did not show such effect (P > 0.05). 4-AP was found to significantly increase the optical density value of the cultured BSMCs (P < 0.01) by MTT method and the numbers of S + G2M BSMCs (P < 0.05) by flow-cytometry. TEA and Glib did not show such effects. 4-AP significantly increased the Ca2+ concentration in cultured BSMCs (P < 0.01). TEA and Glib did not show such effects. CONCLUSION: This result suggests that inhibition of KV activity can increase intracellular Ca2+ and proliferation of rat BSMCs, but inhibition of KCa and KATP showed no effect. |
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