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Park KS, Jung KH, Kim SH, Kim KS, Choi MR, Kim Y, Chai YG: Functional expression of ion channels in mesenchymal stem cells derived from umbilical cord vein. Stem Cells. 2007 Aug;25(8):2044-52. Epub 2007 May 24.
Mesenchymal stem cells have the ability to renew and differentiate into various lineages of mesenchymal tissues. We used undifferentiated human mesenchymal-like stem cells from human umbilical cord vein (hUC-MSCs), a cell line which contains several mesenchymal cell markers. We characterized functional ion channels in cultured hUC-MSCs with whole-cell patch clamp and reverse transcription-polymerase chain reaction (RT-PCR). Three types of outward current were found in these cells: the Ca (2+)-activated K (+) channel (IK (Ca)), a transient outward K (+) current (I (to)), and a delayed rectifier K (+) current (IK (DR)). IK (Ca) and IK (DR) were totally suppressed by tetraethylammonium, and IK (Ca) was sensitive to a specific blocker, iberiotoxin. I (to) was inhibited by 4-aminopyridine. Another type of inward rectifier K (+) current (K (ir)) was also detected in approximately 5% of hUC-MSCs. Elevation of external potassium ion concentration increased the K (ir) current amplitude and positively shifted its reversal potential. In addition, inward Na (+) current (I (Na)) was found in these cells ( approximately 30%); the current was blocked by tetrodotoxin and verapamil. In the RT-PCR analysis, Kv1.1, Kv4.2, Kv1.4, Kir2.1, heag1, MaxiK, hNE-Na, and TWIK-1 were detected. These results suggested that multiple functional ion channel currents, IK (Ca), IK (DR), I (to), I (Na), and K (ir), are expressed in hUC-MSCs. Disclosure of potential conflicts of interest is found at the end of this article. |
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