| 7797053 |
Gusev GP, Sherstobitov AO: Effect of metabolic inhibitors on K+ transport across the lamprey (Lampetra fluviatilis) erythrocyte membrane. Gen Physiol Biophys. 1994 Dec;13(6):459-68.
In order to assess the contribution of oxidative metabolism to K+(86Rb+) transport across the lamprey red cell membrane, the effects of various metabolic inhibitors were examined. The influx of K+ was reduced markedly in the presence of 20 mumol/l 2,4-dinitrophenol (2,4-DNP) or rotenone, and to a lesser extent by 1 mmol/l cyanide. Rotenone produced complete inhibition of the K+ active transport and a partial blockade of K+ channels by 28% on the average. Addition of 2,4-DNP to incubation media resulted in a significant reduction of both active transport of K+ (by 47%) and of K+ movement via channels (by 57%). The inhibitory effect of 2,4-DNP on total K+ influx was independent on decreasing extracellular pHe from 7.4 to 6.5. The blocking action of 1 mmol/l Ba2+ on K+ channels was abolished in the red cells incubated at pHe 6.5. Treatment of the red cells with 1 mmol/l cyanide diminished active transport of K+ to about 34% of control values but did not affect K+ channels. The obtained data indicate that in the lamprey red blood cells at least a half of energy needed for the active transport of K+ is supplied with ATP produced by oxidative phosphorylation. It may be suggested that NADH dehydrogenase is the key enzyme required for active transport of K+ in the cells, as rotenone, a selective blocker of this enzyme, causes a complete blockade of the Na+, K (+)-pump. |
81(1,1,1,1) |