| 15374744 |
Liu X, Stan Leung L: Sodium-activated potassium conductance participates in the depolarizing afterpotential following a single action potential in rat hippocampal CA1 pyramidal cells. Brain Res. 2004 Oct 15;1023(2):185-92.
The depolarizing afterpotential (DAP) following an action potential increases the excitability of a neuron. Mechanisms related to the DAP following an antidromic or current-induced spike were studied in CA1 pyramidal cells by whole-cell recordings in hippocampal slices in vitro. In DAP-holding voltage curves, the DAP at 10 ms after the spike peak (DAP10) was extrapolated to reverse at about -50 mV. Increase of extracellular K (+) concentration increased DAP and neuronal bursting. DAP10 reversal potential shifted positively with an increase in [K (+)](o) and with the blockade of K (+) conductance using pipettes filled with Cs (+). Similarly, extracellular tetraethylammonium (TEA; 10 mM), 4-aminopyridine (3-10 mM) increased DAP and shifted the DAP10 reversal potential to a depolarizing direction. Decrease of [Ca (2+)](o) did not alter DAP significantly, suggesting a nonessential role of Ca (2+) in the DAP. Perfusion of tetrodotoxin (TTX; 0.1-1 microM) and replacement of extracellular Na (+) by choline (+) suppressed both spike height and DAP simultaneously. Replacement of extracellular Na (+) by Li (+) increased DAP and spike bursts, and caused a positive shift of the DAP10 reversal potential. It is suggested that Li (+) increased DAP by blocking an Na (+)-activated K (+) current. In summary, multiple K (+) conductances are normally active during the DAP following a single action potential. |
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