| 12220540 |
Belyaeva EA, Glazunov VV, Korotkov SM: Cyclosporin A-sensitive permeability transition pore is involved in Cd (2+)-induced dysfunction of isolated rat liver mitochondria: doubts no more. Arch Biochem Biophys. 2002 Sep 15;405(2):252-64.
There is dose-dependent Cd (2+)-evoked swelling of isolated rat liver mitochondria energized by complex I, II, or IV respiratory substrates in sucrose medium in the absence of added Ca (2+) and P (i), which is prevented by Sr (2+). Permeability transition effectors (ADP, CsA, EGTA, RR, DTT, ATR, P (i), and Ca (2+)) affect in a corresponding way Cd (2+)-promoted membrane permeabilization in NH (4) NO (3), KCl, and sucrose media. Maximal depression of Cd (2+)-induced swelling is achieved by simultaneous addition of ADP, Mg (2+), and CsA that produces either synergistic (NH (4) NO (3)) or additive (KCl and sucrose media) action. Sustained activation by low [Cd (2+)] of mitochondrial basal respiration in KCl medium is observed both in the absence and in the presence of rotenone and/or oligomycin but only in the latter case (rotenone+oligomycin) CsA inhibits completely Cd (2+) activation of St 4 respiration and partially reverses DNP-uncoupled respiration depressed by cadmium. Cd (2+) effects are discussed in terms of comparison with those of Zn (2+) and PhAsO. |
81(1,1,1,1) |