| 17055466 |
Cogolludo A, Frazziano G, Briones AM, Cobeno L, Moreno L, Lodi F, Salaices M, Tamargo J, Perez-Vizcaino F: The dietary flavonoid quercetin activates BKCa currents in coronary arteries via production of H2O2. Cardiovasc Res. 2007 Jan 15;73(2):424-31. Epub 2006 Sep 20.
Role in vasodilatation.. OBJECTIVE: Large conductance Ca (2+)-activated K (+) channels (BKCa) regulate coronary artery tone in vivo, play a key role in blood pressure regulation, and have been suggested as novel potential drug targets in hypertension. Quercetin exerts systemic and coronary vasodilator effects in vitro and reduces blood pressure in several rat models of hypertension, and its consumption is associated with a lower mortality rate from coronary heart disease in epidemiological studies. We hypothesized that quercetin might activate BKCa channel in isolated myocytes from rat coronary arteries and that this mechanism might be involved in its coronary artery relaxant effects. METHODS: Membrane currents were measured using the whole-cell configuration of the patch-clamp technique. Contractile tension was recorded in rat coronary artery rings mounted in a myograph. RESULTS: Quercetin (> 0.1 muM) increased the outward currents in the whole range of test potentials, hyperpolarized cell membranes, and increased the frequency of spontaneous transient outward currents (STOCs) carried by BKCa channels. These effects were abolished by the selective BKCa blocker iberiotoxin and by catalase. Quercetin increased dichlorofluorescein fluorescence in coronary arteries in a polyethylenglycol-catalase-sensitive manner, indicating that it increased cytosolic H (2) O (2). The membrane-permeable analogue of H (2) O (2) t-butylhydroperoxide mimicked the effects of quercetin on outward currents. The vasodilator effect of quercetin in isolated rat coronary arteries was partially inhibited by iberiotoxin. CONCLUSION: Quercetin increased BKCa currents via production of intracellular H (2) O (2). This effect is involved, at least partly, in the coronary vasodilator effects of quercetin. |
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