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Garait B, Couturier K, Servais S, Letexier D, Perrin D, Batandier C, Rouanet JL, Sibille B, Rey B, Leverve X, Favier R: Fat intake reverses the beneficial effects of low caloric intake on skeletal muscle mitochondrial H (2) O (2) production. Free Radic Biol Med. 2005 Nov 1;39(9):1249-61. Epub 2005 Aug 8.
Food restriction is the most effective modulator of oxidative stress and it is believed that a reduction in caloric intake per se is responsible for the reduced generation of reactive oxygen species (ROS) by mitochondria. Hydrogen peroxide (H (2) O (2)) generation and oxygen consumption (O (2)) by skeletal muscle mitochondria were determined in a peculiar strain of rats (Lou/C) characterized by a self-low-caloric intake and a dietary preference for fat. These rats were fed either with a standard high-carbohydrate (HC) or a high-fat (HF) diet and the results were compared to those measured in Wistar rats fed a HC diet. H (2) O (2) production was significantly reduced in Lou/C rats fed a HC diet; this effect was not due to a lower O (2) consumption but rather to a decrease in rotenone-sensitive NADH-ubiquinone oxidoreductase activity and increased expression of uncoupling proteins 2 and 3. The reduced H (2) O (2) generation displayed by Lou/C rats was accompanied by a significant inhibition of permeability transition pore (PTP) opening. H (2) O (2) production was restored and PTP inhibition was relieved when Lou/C rats were allowed to eat a HF diet, suggesting that the reduced oxidative stress provided by low caloric intake is lost when fat proportion in the diet is increased. |
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