| 4052077 |
Barr R, Sandelius AS, Crane FL, Morre DJ: Oxidation of reduced pyridine nucleotides by plasma membranes of soybean hypocotyl. Biochem Biophys Res Commun. 1985 Sep 16;131(2):943-8.
Highly purified plasma membranes isolated from soybean hypocotyls by free-flow electrophoresis or by a two-phase polymer separation system oxidize reduced pyridine nucleotides, NADH or NADPH, at rates of 2-5 nanomoles/mg protein/min. These rates are not influenced by mitochondrial inhibitors or by inhibitors of the alternate respiratory pathway. The NADH oxidase has a Km of 200 microM NADH. The enzyme activity is stimulated by Ca2+ and Mg2+ ions. The function of this enzyme is unknown at present, but it may represent a redox-controlled proton pump linked to acidification. |
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