| 18766020 |
Gao XF, Wang W, He C: Rotenone inhibits delayed rectifier K+ current via a protein kinase A-dependent mechanism. Neuroreport. 2008 Sep 17;19(14):1401-5.
Voltage-gated K+ channels (K (V)) regulate cell electrical properties, proliferation, migration, and death. Rotenone is a mitochondrial inhibitor, influencing activity of many channels that potentially participate in cell death processes, but its effect on K (V) channel in neurons remains unclear. This study used whole-cell patch clamp methods and found that rotenone concentration dependently decreased delayed rectifier K+ current (I (DR)) amplitude in cultured ventral mesencephalic neurons, but had no effect on A-type current (I (A)) peak amplitude. Furthermore, the protein kinase A inhibitor H-89 prevented rotenone-induced decrease in I (DR). The inhibition of I (DR) by rotenone was mimicked by extracellular application of forskolin. In summary, our results indicate that rotenone acutely decreased I (DR) currents in cultured mesencephalic neurons via a protein kinase A-dependent mechanism. |
143(1,3,3,3) |