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Salin ML, Bridges SM: Chemiluminescence in Wounded Root Tissue : EVIDENCE FOR PEROXIDASE INVOLVEMENT. Plant Physiol. 1981 Jan;67(1):43-46.
Root and stem segments from soybean (Merrill cv. ;Bragg') showed an enhanced chemiluminescence upon mechanical injury. Roots emitted more light than did stems. Light emission was diminished by CN (-) and N (3) (-) but was not affected by rotenone and antimycin A. Catalase quenched chemiluminescence in wounded root segments as did ascorbic acid and hydroquinone. Superoxide dismutase addition resulted in a small diminution in light emission, but mannitol, an OH. scavenger, was without effect. The addition of H (2) O (2) to wounded root segments markedly elevated chemiluminescence in the presence of air as well as under N (2). It is concluded that peroxidases, found abundantly in roots, predominantly contribute to light emission in wounded plant tissue. |
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