| 11095953 |
Murphy HS, Yu C, Quddus J: Functional expression of NAD (P) H oxidase p47 in lung microvascular endothelial cells. Biochem Biophys Res Commun. 2000 Nov 30;278(3):584-9.
Vascular endothelial cell superoxide (O (*)(2)) has an important role in intracellular signaling, in interaction with other reactive species such as nitric oxide, and in vascular dysfunction. Little is known regarding the source and function of O (*)(2) from microvascular endothelial cells from specific tissues. Mouse lung microvascular endothelial cells stimulated with phorbol ester (PMA) or NADPH generated significant O (*)(2), which was inhibited by diphenyleneiodonium (DPI) but not by allopurinol, rotenone, indomethacin, or quinacrine. Optimal O (*)(2) generation required cytosolic as well as particulate cell fractions of cells. In parallel studies, PMA induced increased expression of the p47 component of the NAD (P) H oxidase in the particulate fraction, which was inhibited by staurosporine and calphostin. These data demonstrate that NAD (P) H oxidase is an important source of O (*)(2) generation in lung microvascular endothelial cells. |
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