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Fitzgerald EM, Djamgoz MB: Sensitivity of valinomycin-based K (+)-selective micro-electrodes to inhibitors of K+ transport. J Neurosci Methods. 1995 Jul;59(2):273-7.
The sensitivies of double-barrelled K (+)-selective micro-electrodes (KSMs) employing the low-impedance membrane cocktail based on the neutral K (+)-selective ion carrier valinomycin (Fluka, Cocktail B 60398) to the following 3 different classes of inhibitors of K+ transport were measured: (1) general metabolic inhibitors (dinitrophenol, potassium cyanide, sodium azide, rotenone, dicyclohexylcarbodiimide, salicylhydroxamic acid); (2) P-type ATPase inhibitors (vanadate, ouabain, amiloride, SCH 28080); and (3) anion-dependent K+ transport inhibitors (bumetanide, 4-acetamide-4-isothiocyanostilbene-2,2-disulphonic acid). Of the 12 inhibitors tested, only dinitrophenol had any significant effect on the response of KSMs to K+ activity. Comparison of the calibrations in solutions with and without 0.1 mM dinitrophenol showed that this inhibitor behaved as a 'classical' interferent whereby its contribution to the K+ activity signal was statistically significant at K+ activities of 36.0 mM and less. However, at higher K+ activities (97.0 mM), dinitrophenol interference was not significant. It was possible to correct for the DNP interference and to obtain measurements of intracellular K+ activity in insect muscles. |
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