| 171520 |
Ryrie IJ: Studies of the oligomycin-sensitive ATPase from yeast mitochondria. J Supramol Struct. 1975;3(3):242-7.
Reconstitution of ATP-32Pi exchange in the presence of phospholipids.. A purified preparation of the oligomycin-sensitive ATPase from yeast mitochondria has been shown to elicit an oligomycin- and uncoupler-senstitive ATP-32Pi exchange in the presence of phospholipids. Reconstitution was normally achieved by dialysis of an ATPase-phospholipid-cholate mixture. Following this procedure, vesicles with diameters between 200 and 1,500 A were seen by electron microscopy. As in mitochondria, ATPase activity in the reconstituted system was stimulated by a range of uncouplers which inhibited ATP-32Pi exchange. These and other findings suggest that the coupling mechanism may still be intact within the ATPase complex. |
5(0,0,0,5) |