| 14638429 |
Palese LL, Gaballo A, Technikova-Dobrova Z, Labonia N, Abbrescia A, Scacco S, Micelli L, Papa S: Characterization of plasma membrane respiratory chain and ATPase in the actinomycete Nonomuraea sp. FEMS Microbiol Lett. 2003 Nov 21;228(2):233-9.
ATCC 39727.. We have characterized the respiratory system of the aerobic actinomycete Nonomuraea sp. ATCC 39727. The plasma membrane of the microorganism is shown to contain a protonmotive respiratory chain and H+-ATPase. The respiratory chain is made up of a rotenone-sensitive NADH-quinone oxidoreductase, a four subunits aa3-type cytochrome c oxidase and a bc1 complex. The H+-ATPase is characterized as an F0F1-type on the basis of its sensitivity to specific inhibitors; the enzyme is also inhibited by mM concentrations of Ca2+. The activity of the respiratory chain increases during the exponential growth phase, but is depressed in the stationary phase. The H+-ATPase activity reaches, as the respiratory chain, a maximal activity at the end of the exponential growth phase and then remains constant in the stationary phase. |
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