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Tarshis MA, Fedotov NS: [Basic evidence for the active transport of carbohydrates in the membrane vesicles of Acholeplasma laidlawii cells]. Biofizika. 1977 Mar-Apr;22(2):275-80. A. laidlawii membrane vesicles are able to accumulate C14-glucose as well as maltose and fructose against the concentration gradient in the absence of exogeneous entergetic sources. Sugar transport is inhibited by anaerobiosis and by the electron transfer inhibitors such as rotenone and amytal, and by proton conductors such as carbonylcyanide-m-chlorophenylhydrazone. Arsenate and dicyclohexylcarbodimide (inhibitor of membrane-bound ATPase) do not inhibit the sugar transport. It is concluded that sugar transport in the membrane vesicles can be driven by the high-energy state of the membrane or the membrane potential. |
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