| 16876113 |
Deng XL, Sun HY, Lau CP, Li GR: Properties of ion channels in rabbit mesenchymal stem cells from bone marrow. Biochem Biophys Res Commun. 2006 Sep 15;348(1):301-9. Epub 2006 Jul 20.
The present study was designed to investigate properties of ion channels in undifferentiated rabbit mesenchymal stem cells (MSCs) from bone marrow using whole-cell patch-clamp and RT-PCR techniques. It was found that three types of outward currents were present in rabbit MSCs, including an inward rectifier K (+) current (I (Kir)), a noise-like Ca (2+)-activated K (+) current (I (KCa)) co-present with delayed rectifier K (+) current (IK (DR)). I (Kir) was inhibited by Ba (2+), while I (KCa) was inhibited by paxilline (a blocker of big conductance I (KCa) channels) and clotrimazole (an inhibitor of intermediate conductance I (KCa) channels). IK (DR) exhibited a slow inactivation, "U-shaped" voltage-dependent inactivation, and slow recovery from inactivation, and the current was inhibited by tetraethylammonium or 4-aminopyridine. RT-PCR revealed the molecular identities for the functional ionic currents, including Kir1.1 (possibly responsible for I (Kir)), KCa1.1 and KCa3.1 (possibly responsible for I (KCa)), and Kv1.2, Kv2.1, and Kv2.2 (possibly responsible for IK (DR)). These results demonstrate for the first time that three types of functional ion channel currents (i.e., I (Kir), I (KCa), and IK (DR)) are present in rabbit MSCs from bone marrow. |
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