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Armstrong DJ, Murai N, Taller BJ, Skoog F: Incorporation of Cytokinin N-Benzyladenine into Tobacco Callus Transfer Ribonucleic Acid and Ribosomal Ribonucleic Acid Preparations. Plant Physiol. 1976 Jan;57(1):15-22.
The incorporation of the cytokinin N (6)-benzyladenine into tobacco (Nicotiana tabacum) callus tRNA and rRNA preparations isolated from tissue grown on medium containing either N (6)-benzyladenine-8-(14) C or N (6)-benzyladenine-8-(14) C: benzene-(3) H (G) has been examined. N (6)-benzyladenine was incorporated into both the tRNA and rRNA preparations as the intact base. Over 90% of the radioactive N (6)-benzyladenosine recovered from the RNA preparations was associated with the rRNA. Purification of the crude rRNA by either MAK chromatography or Sephadex G-200 gel filtration had no effect on the N (6)-benzyladenosine content of the RNA preparation. The distribution of N (6)-benzyladenosine moieties in tobacco callus tRNA fractionated by BD-cellulose chromatography did not correspond to the distribution of ribosylzeatin activity. N (6)-benzyladenosine was released from the rRNA preparation by treatment with venom phosphodiesterase and phosphatase, ribonuclease T (2) and phosphatase, or ribonuclease T (2) and a 3'-nucleotidase. N (6)-benzyladenosine was not released from the RNA preparation by treatment with either ribonuclease T (2) or phosphatase alone or by successive treatment with ribonuclease T (2) and a 5'-nucleotidase. Brief treatment of the rRNA preparation with ribonuclease T (1) and pancreatic ribonuclease converted the N (6)-benzyladenosine moieties into an ethyl alcohol soluble form. On the basis of these and earlier results, the N (6)-benzyladenosine recovered from the tobacco callus RNA preparations appears to be present as a constituent of RNA and not as a nonpolynucleotide contaminant. |
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