| 19607946 |
De Giglio E, Cometa S, Ricci MA, Zizzi A, Cafagna D, Manzotti S, Sabbatini L, Mattioli-Belmonte M: Development and characterization of rhVEGF-loaded poly (HEMA-MOEP) coatings electrosynthesized on titanium to enhance bone mineralization and angiogenesis. Acta Biomater. 2010 Jan;6(1):282-90. Epub 2009 Jul 14.
Osteointegration of titanium implants could be significantly improved by coatings capable of promoting both mineralization and angiogenesis. In the present study, a copolymeric hydrogel coating, poly-2-hydroxyethyl methacrylate-2-methacryloyloxyethyl phosphate (P (HEMA-MOEP)), devised to enhance calcification in body fluids and to entrap and release growth factors, was electrosynthesized for the first time on titanium substrates and compared to poly-2-hydroxyethyl methacrylate (PHEMA), used as a blank reference. Polymers exhibiting negatively charged groups, such as P (HEMA-MOEP), help to enhance implant calcification. The electrosynthesized coatings were characterized by X-ray photoelectron spectroscopy and atomic force microscopy. MG-63 human osteoblast-like cell behaviour on the coated specimens was investigated by scanning electron microscopy, MTT viability test and osteocalcin mRNA detection. The ability of negatively charged phosphate groups to promote hydroxyapatite-like calcium phosphate deposition on the implants was explored by immersing them in simulated body fluid. Similar biological responses were observed in both coated specimens, while calcium-phosphorus globules were detected only on P (HEMA-MOEP) surfaces pretreated with alkaline solution. Testing of the ability of P (HEMA-MOEP) hydrogels to entrap and release human recombinant vascular endothelial growth factor, to tackle the problem of insufficient oxygen and nutrient delivery, suggested that P (HEMA-MOEP)-coated titanium prostheses could represent a multifunctional material suitable for bone restoration applications. |
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