Protein Information

ID 27
Name cytochrome c
Synonyms CYC; CYCS; Cytochrome C; HCS; Cytochrome Cs

Compound Information

ID 1802
Name piperonyl butoxide
CAS 5-[[2-(2-butoxyethoxy)ethoxy]methyl]-6-propyl-1,3-benzodioxole

Reference

PubMed Abstract RScore(About this table)
7864625 Kato H, Kodama O, Akatsuka T: Characterization of an inducible P450 hydroxylase involved in the rice diterpene phytoalexin biosynthetic pathway. Arch Biochem Biophys. 1995 Feb 1;316(2):707-12.
Ent-isopimara-8 (14),15-dien-3 beta-ol (1) was isolated from uv-irradiated rice (Oryza sativa L.) leaves. Since 1 was converted to the rice diterpene phytoalexins and oryzalexins D (ent-isopimara-8 (14),15-diene-3 beta,7 alpha-diol) and E (ent-isopimara-8 (14),15-diene-3 beta,9 alpha-diol) in uv-irradiated rice leaf microsome fraction in the presence of oxygen and NADPH, it was concluded that 1 was the biosynthetic precursor of these oryzalexins. This enzyme reaction was inhibited by cytochrome P450 inhibitors such as piperonyl butoxide, SKF-525A, paclobutorazole, metyrapone, cytochrome c, and carbon monoxide. The lack of inhibitory activity of 1 for spore germination of Pyricularia oryzae indicates that the final cytochrome P450-dependent hydroxylation steps in converting 1 to oryzalexins D and E are essential for the production of rice phytoalexins with antifungal activities.
31(0,1,1,1)